HPLC法测定注射用炎琥宁中脱水穿心莲内酯琥珀酸半酯的含量

目的建立HPLC法测定注射用炎琥宁中脱水穿心莲内酯琥珀酸半酯的含量。方法色谱柱watersC18（4．6μm×150μm），乙腈-0．05mol·mL-1的磷酸二氢钾溶液（35：65）为流动相．流速为1．OmL·min-1，检测波长251nm。结果实验结果表明。脱水穿心莲内酯琥珀酸半酯在39．87μmg·mL-1 -398．70μmg·mL-1。范围内与峰面积呈良好的线性关系（r=O．9999），平均回收率为100．34％，RSD=0．25％（n=9）。结论该方法简便快速，重现性好，可用于炎琥宁的质量控制。

Determination of dehydroandrographolide succinate in potassium sodium pehydroandrographolide succinate for injection by HPLC

OBJECTIVE To establish a HPLC method for detemination of dehydroandrographolide succinate in potassium sodium pehydroandrographolide succinate for injection. METHODS The waters Ci8（4.6μm× 150μm） was used, the mobile phase consisted of acetoniteile-0.05mol· mL- 1 potassiun dihydrogen phosphate（35 ： 65） with flow rate of 1.0mL· min-1. The detection wavelength was 251nm. RESULTS The method was linear with in the range of 39.87μg· mL- 1 - 398.70μg· mL- 1 （r = 0. 9999） . The average recovery was 100.34 %, and RSD was 0.25 % （n = 9）. CONCLUSION The method is efficient and accurate with a good repeatability and can be using for the control of potassium sodium pehydroandrographolide succinate for injection.