肝癌细胞稳定转染B7.1后的免疫学特性

目的 探讨赋予肝癌细胞第二信号分子Ｂ７．１增强癌细胞与淋巴细胞之间的识别与激活作用，从而达到增强淋巴细胞对肝癌细胞的杀伤或抑制作用。方法 利用逆转录方法，转染Ｂ７．１分子至包装细胞系ＰＡ３１７。筛选获得高滴度的克隆后，以病毒上清感染肝癌细胞，使其表达Ｂ７．１分子，最后以ＬＤＨ释放法测定ＬＡＫ细胞的细胞毒活性。结果 肝癌细胞可稳定表达Ｂ７．１分子，阳性率达９４．３％，未转染的细胞无表达，其所诱发的Ｌ

Pilot study of immunoreactivity on B7.1 gene transfecting hepatocarcinoma cells

Aim To study costimulation mediated by costimulatory molecules, B7 1, counter receptor ligands for the CD28/cytolytic T lymphocyte associated antigen(CTLA) 4, which plays an important role in the induction of T cell mediated antitumor immunity against HCC. Methods Retroviral transduction was applied in transfecting pLXSN B7.1 into packaging cell line,PA317, and high titer clone was acquired. Then the virus stock were used to infect the HHCC cell lines and the clone strongly expressing B7.1 was picked out. At last the primary LAK cytotoxicity were processed through the method of LDH release. Results After transfection, HHCC cell line could express B7.1 steadily. The introduction of B7.1 into HHCC cells in vitro induced a strong immunity that was associated with LAK cells in contrast to the untransfected cells in which B7.1 was not expressed (P<0.01). Conclusion Our findings suggest that B7 1 gene transfer is an appropriate way to induce strong expression of this molecule and enhance the recognition by LAK cells. This might be useful for studying the B7.1 molecular more thoroughly and for immuno gene therapy against human HCC.