汉坦病毒核蛋白基因片段真核表达载体的构建及鉴定

目的 确定特定小鼠ＭＨＣ Ｉ类分子Ｈ－２ｄ限制性另工提呈的汉坦病毒核蛋白（ＮＰ）抗原表位。方法 在计算机预测的基础上，以编码ＮＰ的Ｓ基因为模板，合成了３个相互重叠的基因片段，构建了以携带ＧＦＰ报告基因的ｐＥＧＦＰ－Ｎ２为持载体的重组质粒，并进行了初步表达及鉴定。结果 目的基因片段已插入质粒载体，并在转染的真核细胞内成功表达。结论 汉坦病毒核蛋白片段能够被细胞加工提呈。

Construction and identification of eukaryotic expression vector bearing segments of Hataan virus nucleocapsid protein

Aim To identify the exact epitopes of Hantaan virus nucleocapsid protein(NP) presented by murine MHC class I molecule, H 2 d. Methods We amplified three overlapping gene segments based on computer epitope predicting software, using the viral genome S encoding NP as template. These three segments were inserted into eukaryotic expression vector pEGFP N2. Results The constructs were successfully inserted and expressed. Conclusion The segments can be presented by the transfected cells and the method is a useful tool for epitope determination.