X盒结合蛋白1反应产物在局灶性脑缺血后内源性神经干细胞增殖过程中的作用

背景:细胞核转录因子X盒结合蛋白1在中枢神经系统发育中表达,对神经元的增殖都有很重要的作用。目的:检测细胞核转录因子X盒结合蛋白1在大鼠脑缺血再灌注海马组织神经干细胞中的表达及碱性成纤维细胞生长因子的干预作用。方法:采用大脑中动脉栓塞制作大鼠脑缺血再灌注模型。大鼠随机分为对照组、缺血再灌注组、碱性成纤维细胞生长因子组,免疫组织化学法检测海马神经干细胞BrdU、X盒结合蛋白1的表达及碱性成纤维细胞生长因子的干预作用。结果与结论:脑缺血再灌注第3天缺血海马神经元BrdU阳性细胞明显增多,第7天达高峰。X盒结合蛋白1反应产物第3天较对照组增多,随缺血再灌注时间的延长逐渐增多,第7天达高峰,以后表达逐渐减少。说明碱性成纤维细胞生长因子促进神经干细胞的增殖及缺血脑组织X盒结合蛋白1的表达,其促进神经干细胞增殖作用可能由X盒结合蛋白1信号介导。

Effect of X box-binding protein 1 on the proliferation of endogenous neural stem cells after focal cerebral ischemia

BACKGROUND:Nuclear transcription factor X box-binding protein 1(Xbp1) is expressed during the development of the central nervous system,and plays an important role in neurons cell proliferation.OBJECTIVE:To investigate the expression of nuclear transcription factor Xbp1 in neural stem cells of cerebral ischemia/reperfusion hippocampal tissue and the inhibition effects of basic fibroblast growth factor(bFGF).METHODS:A novel model of cerebral ischemia/reperfusion was established with the method of middle cerebral artery occlusionin rats.The rats were divided into control group,ischemia/reperfusion group and bFGF group.The expression of BrdU and Xbp1 and the inhibition effects of bFGF in hippocampal neural stem cells were detected with immunohistochemical method.RESULTS AND CONCLUSION:After 3 days,BrdU positive cells in the hippocampus were obviously increased.To the 7th day,BrdU positive cells were more than those at any time.At 3 days,Xbp1 reaction products in ischemia/reperfusion were more than that in control group,and reached the peak on day 7,and then gradually decreased.bFGF can promotes the expression of Xbp1 in the hippocampus tissue and the proliferation of neural stem cells.These findings indicate that bFGF promotion of neural stem cells proliferation may be mediated by Xbp1 signaling pathway.