芦荟多糖对脊髓缺血损伤的神经保护作用

目的:探讨芦荟多糖(aloe polysaccharide,AP)对兔脊髓缺血损伤是否有神经保护作用.方法:32只成年雄性新西兰兔随机分成4组(每组8只 ),即对照组(C 组)、芦荟多糖组(A组)、溶剂对照组( V组 ) 及假手术组(S组).A组在脊髓缺血前30min经耳缘静脉给予50m·kg-1芦荟多糖;V组以同样方式给予等容量生理盐水;C组仅仅制备脊髓缺血损伤模型,不进行其它处理;S组仅仅暴露腹主动脉,而不阻断它,其他处理同C组;兔脊髓缺血模型采用夹闭兔腹主动脉肾下段20min.再灌注后48h,对所有动物神经功能评分,然后处死动物取脊髓(L5-7),制作标本行组织病理学观察.结果:A组的神经功能评分和脊髓前角正常神经细胞数明显多于C组及V组(P<0.01);C组及V组的神经功能评分和脊髓前角正常神经细胞数组间无明显差异(P>0.05);神经功能评分与其对应脊髓前角正常神经细胞计数之间有显著相关性(r=0.804,P<0.01).结论:芦荟多糖对兔脊髓缺血再灌注损伤有明显的神经保护作用.

Neuroprotective effect of aloe polysaccharide against spinal cord ischemic injury in rabbits

Objective To investigate whether aloe polysaccharide has neuroprotective effect against spinal cord ischemic injury in rabbits.Methods 32 male New Zealands rabbits were randomly assigned to 4 groups(8 in each group) as follows: The animals in control group(C)only underwent spinal cord ischemia without pretreatment The animals in aloe polysaccharide group(A) received intravenous infusion of 50m·kg-1 aloe polysaccharide(dissolved in normal saline) 30 min before spinal cord ischemia.The animals in vehicle group(V) received intravenous infusion of the same volume normal saline 30 min before spinal cord ischemia;The animals in sham group underwent only the exposure of the aorta.The spinal cord ischemia was induced for 20min by infrarenal aortic occlusion.Neurologic status was scored at 48 h after reperfusion.Then,all animals were sacrificed and the spinal cords(L5-7) were removed for histopathologic study.Rerults The neurologic function scores and the number of normal neurons of anterior horn of spinal cord in A group were significantly greater than those in C group and V group(P<0.01).The neurologic function scores and the number of normal neurons of anterior horn of spinal cord among C group and V group had no statistical differences(P>0.05).There was a stong correlation between the neurologic function scores and the number of normal neurons in anterior horn of spinal cord(r= 0.804,P<0.01).Conclusion Aloe polysaccharide has a significant neuroprotective effect against spinal cord ischemic injury in rabbits.