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| 皮肤间充质干细胞的原代培养 | |
| 引用本文： | 潘智慧,王丽,刘瑞风,张永翠,李欣,王荣,刘小民,张开明. 皮肤间充质干细胞的原代培养[J]. 中国皮肤性病学杂志, 2012, 26(2): 114-117 |
| 作者姓名： | 潘智慧 王丽 刘瑞风 张永翠 李欣 王荣 刘小民 张开明 |
| 作者单位： | 1. 山西医科大学第二医院,山西太原,030001 2. 太原市中心医院,山西太原,030009 3. 中北大学医院大内科,山西太原,030051 |
| 基金项目： | 国家自然科学基金资助项目，山两省级自然科学基金资助项目 |
| 摘 要： | 目的评估体外分离培养皮肤间充质干细胞(sMSCs)的适宜方法。方法采用低血清培养和无血清培养两种不同的原代培养法,体外培养皮肤间充质干细胞,通过观察细胞形态特征及流式细胞技术进行鉴定,比较其差异。结果两种培养法均能培养出形态均一、成熟的皮肤间充质干细胞,但是低血清法培养细胞所用时间8.93d明显小于无血清培养法所需时间15.93d,差异有统计学意义(P<0.001),且低血清法培养出的细胞较无血清培养方法得到的皮肤间充质干细胞的CD44阳性率较高,CD45阴性率较低,差异有统计学意义(P<0.001)。结论低血清培养法是一种适用于临床试验研究皮肤间充质干细胞的原代培养方法。 |
| 关 键 词： | 间充质干细胞 皮肤 体外培养 |
Primary Cultured Mesenchymal Stem Cells from Skin |
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| PAN Zhi-hui,WANG Li,LIU Rui-feng,ZHANG Yong-cui,LI Xin,WANG Rong,LIU Xiao-min,ZHANG Kai-ming. Primary Cultured Mesenchymal Stem Cells from Skin[J]. The Chinese Journal of Dermatovenereology, 2012, 26(2): 114-117 | |
| Authors: | PAN Zhi-hui WANG Li LIU Rui-feng ZHANG Yong-cui LI Xin WANG Rong LIU Xiao-min ZHANG Kai-ming |
| Affiliation: | 1.Department of Dermatology,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China;2.Department of Dermatology,Taiyuan City Hospital,Taiyuan 030009,China;3.Department of Inter Medicine,Hospital of Zhongbei University,Taiyuan 030051,China) |
| Abstract: | Objective To evaluate the suitable method to isolate mesenchymal stem cells from the skin in vitro.Methods Two different methods low serum and serum-free primary culture were adopted to culture mesenchymal stem cells from the skin in vitro.The differences were compared by observing cell morphology under microscope and using flow cytometry techonology.Results Both two methods could culture mature mesenchymal stem cells from skin.But the cultured time,which in low serum group was 8.93 d while in serum-free group was 15.93 d,had statistically significant(P<0.001).Compared with serum-free culture method,low serum cells cultured cells with higher positive rate of CD44 and lower negative rate of CD45,which had statistically significant(P<0.001).Conclusion Low serum culture is a method to culture skin mesenchymal stem cells,suitable for clinical trial and research. |
| Keywords: | Mesenchymal stem cells Skin Culture in vitro |
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