Serum granulocyte colony-stimulating factor in patients with chronic renal failure

Objective To gain a better understanding of the regulatory mechanism and kinetic behaviour of granulocyte colony-stimulating factor (G-CSF).Methods An enzyme-linked immunosorbent assay (ELISA) method was used to detect serum G -CSF in 61 patients with chronic renal failure±long-term hemodialysis and 30 normal controls. Results Serum G-CSF levels in CRF patients were significantly higher than in normal con tro ls. Eighty percent of patients had detectable G-CSF and serum G-CSF levels we re 566.40±207.98 ng/L in non-hemodialyzed (non-HD) patients. The detectab le percentage in hemodialyzed patients was 93.33%, serum G-CSF levels in pre-HD and post-HD patients were 1255.36±611.25 ng/L and 1151.61±599 .47 ng/L respectively. Serum G-CSF levels in HD patients were slightly highe r than in non-HD patients, but no significant difference was found between the two groups. No difference was found between the G-CSF values obtained in pre- HD and post-HD patients. There was no relationship between G-CSF levels a nd WBC, BUN or Scr (P>0.05).Conclusion The high value of G-CSF in patients with CRF may be caused by a decrease in G -CSF clearance and/or an increase in G-CSF release.