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Modulation of BmKAS-1 and BmK1-3-2 to sodium channel in rat dorsal root ganglion neurons |
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| Authors: | XIAO Hang Mao Xia Tan Zhiyong SHI Yun ZHAO Zhiqi Ji Yonghua |
| Institution: | Department of Neurotoxicology, Applied Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China;Department of Neurotoxicology, Applied Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China;Department of Neurotoxicology, Applied Institute of Toxicology, Nanjing Medical University, Nanjing 210029, China;Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China;Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China;Shanghai Institute of Physiology, Chinese Academy of Science, Shanghai 200031, China |
| Abstract: | OBJECTIVE: To investigate what effects BmKAS-1 (a polypeptide purified from the Chinese scorpion Buthus martensi Karsch BmK] and named as BmK activator of skeletal-muscle ryanodine receptor) and its upstream mixture BmK1-3-2 have on Na+ channels in dorsal root ganglion (DRG) small diameter neurons. METHODS: The whole-cell patch-clamp technique was used to investigate the effects of BmKAS-1 and BmK1-3-2 on Na+ current in rat small diameter DRG neurons. RESULTS: About 50% peak Na+ current was suppressed by 10 micrograms/ml of BmK1-3-2. 1.62 micrograms/ml of BmKAS-1 also blocked 50% peak Na+ current, and there was an obvious dose-dependent relationship. CONCLUSION: Both BmK1-3-2 and BmKAS-1 have a blocking effect on Na+ channels, and this may one of the mechanisms for the analgetic effect of BmK1-3-2 and BmKAS-1. |
| Keywords: | patch-clamp BmKAS-1 BmK1-3-2 dorsal root ganglion sodium channel |
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