Detection of human herpesvirus 8 DNA in acute leukemia patients

Objective To determine the prevalence of human herpesvirus 8 (HHV-8) DNA in acute leukemi a (AL) patients.Methods The presence of HHV-8 DNA sequences in peripheral blood mononuclear cells (PBMC ) and bone marrow mononuclear cells (BMMC) from 50 AL patients was examined usin g polymerase chain reaction (PCR).Nine human hematopoietic cell lines and PBMC from 30 normal donors were also included.Results HHV-8 DNA sequences were detected in one case of acute myelogenous leukemia (AM L).The specimens from the bone marrow aspirate, peripheral blood and serum of this patient were all positive.None of the normal donors and human hematopoiet ic cell lines showed evidence of HHV-8 DNA.Conclusion The results suggest that the prevalence of HHV-8 is low in AL in China.