Antineoplastic mechanism of Octreotide actionin human hepatoma |
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Authors: | CHEN Xujun Liu Zhisu AI Zhongli |
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Institution: | Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, China;Department of General Surgery, Zhongnan Hospital, Wuhan University, Wuhan 430071, China |
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Abstract: | OBJECTIVES: To investigate whether apoptosis can be induced by Octreotide in human hepatoma cells in vitro and elucidate the antineoplastic mechanism of Octreotide in hepatoma. METHODS: A cultured human hepatoma cell line, BEL-7402, was exposed to Octreotide and apoptosis was evaluated by cytochemical staining (Hochesst 33,258), transmission electron microscopy, agarose gel electrophoresis and flow cytometry (FCM). RESULTS: After exposure to 0.2 microgram/ml Octreotide, apoptosis with nuclear chromatin condensation as well as fragmentation, cell shrinkage and the formation of apoptotic bodies was observed using cytochemical staining and transmission electron microscopy. A DNA ladder in agarose gel electrophoresis was also displayed. FCM showed that the apoptotic cell number rose with an increase in the concentration of Octreotide (0-2 micrograms/ml). There was a positive correlation between Octreotide concentration and apoptotic rate in BEL-7402 cells (r = 0.809, P < 0.05). CONCLUSION: Apoptosis in human hepatoma cells can be induced by Octreotide, which may be related to the mechanism of antineoplastic action of Octreotide in hepatoma. |
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Keywords: | Octreotide liver neoplasm apoptosis in vitro antineoplasticmechanism |
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