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| Analysis of hypermutation of the 5’ noncoding region in the BC L-6 gene | |
| 引用本文： | 王林,刘建湘,黄薇,王辉东,王建民,顾柏炜,陈竺,陈赛娟,许良中,杨文涛,金晓龙,柳红.Analysis of hypermutation of the 5’ noncoding region in the BC L-6 gene[J].中华医学杂志(英文版),2001,114(3):286-290. |
| 作者姓名： | 王林 刘建湘 黄薇 王辉东 王建民 顾柏炜 陈竺 陈赛娟 许良中 杨文涛 金晓龙 柳红 |
| 作者单位： | 1. 上海第二医科大学附属瑞金医院 上海血液学研究所 上海 200025 2. 上海医科大学附属肿瘤医院分子病理研究室 上海 200025 3. 上海第二医科大学附属瑞金医院病理科 上海 200025 4. 徐州医学院病理教研室 徐州 221002 |
| 基金项目： | ThisworkwassupportedbythegrantsfromtheNationalNaturalScienceFoundationofChina (No 3 9770 83 0 )，andDr ClydeFoundation |
| 摘 要： | Objective To investigate BCL-6 gene mutations in Chinese populations with B-cell non- Hodgkin’s lymphoma.Methods Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5’ noncoding re gion of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma ( DLCL) and follicular lymphoma (FL).Results Nine cases were found to have base substitutions.The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% an d (0.56-1.10)×10(-2)/bp, respectively.Conclusions The 5’ regulatory region of the BCL-6 gene undergoes frequent somatic hypermuta tion during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL. |
| 关 键 词： | BCL-6 gene 5’ noncoding region hypermutation diffuse large-cell lymphoma follicular lymphoma |
Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene |
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| WANG Lin,XU Liangzhong,JIN Xiaolong,LIU Jianxiang,HUANG Wei,WANG Huidong,WANG Jianmin,GU Baiwei,YANG Wentao,LIU Hong,CHEN Saijuan.Analysis of hypermutation of the 5' noncoding region in the BCL-6 gene[J].Chinese Medical Journal,2001,114(3):286-290. | |
| Authors: | WANG Lin XU Liangzhong JIN Xiaolong LIU Jianxiang HUANG Wei WANG Huidong WANG Jianmin GU Baiwei YANG Wentao LIU Hong CHEN Saijuan |
| Institution: | Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Department of Molecular Pathology, Tumor Hospital, Shanghai Medical University, Shanghai 200025, China;Department of Pathology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China;Department of Molecular Pathology, Tumor Hospital, Shanghai Medical University, Shanghai 200025, China;Department of Pathology, Xuzhou Medical College, Xuzhou 221002, China;Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical Uni versity, Shanghai 200025, China |
| Abstract: | OBJECTIVE: To investigate BCL-6 gene mutations in Chinese populations with B-cell non-Hodgkin's lymphoma. METHODS: Polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE) and direct DNA sequencing were used to identify mutations in the 5' noncoding region of the BCL-6 gene in a total of 40 cases of diffuse large-cell lymphoma (DLCL) and follicular lymphoma (FL). RESULTS: Nine cases were found to have base substitutions. The incidence of BCL-6 gene mutation and the frequency of single-base changes were approximately 25.7% and (0.56-1.10) x 10(-2)/bp, respectively. CONCLUSIONS: The 5' regulatory region of the BCL-6 gene undergoes frequent somatic hypermutation during lymphomagenesis and the identification of BCL-6 gene hypermutations provides a molecular marker for confirmatory diagnosis of B-NHL. |
| Keywords: | BCL-6 gene 5' noncoding region hypermutation diffuse large-cell lymphoma follicular lymphoma |
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