高效液相色谱荧光法测定大鼠静脉注射桂利嗪的血药浓度

目的:建立大鼠血浆中桂利嗪浓度的高效液相色谱荧光检测法,并研究桂利嗪注射液在大鼠体内的药动学。方法:6只大鼠颈静脉插管给予桂利嗪注射液3mg·kg-1,于给药后5、10、20、40、60、120、240min时静脉取血,采用高效液相色谱荧光法测定血药浓度,并计算主要药动学参数。结果:桂利嗪检测浓度线性范围为10～1000ng·mL-(1r=0.9996),最低检测限为0.2ng·mL-1,平均提取回收率为95.9%～98.6%,RSD<8.6%。t1/2为(87.73±22.54)min,AUC0～240min为(58453±8672.8)ng·min·mL-1。结论:该法简便、准确、灵敏,适用于桂利嗪的药动学研究。

Determination of Plasma Concentration in Rats after Intravenous Injection Cinnarizine by HPLC-FLU

OBJECTIVE： To develop an HPLC-FLU method for the determination of cinnarizine in rat plasma and to study its pharmacokinetics. METHODS： 6 rats were given cinnarizine injection （3 mg·kg^-1） by venous cannula and after 5 min, 10 min, 20 min, 40 min, 60 min, 120 min and 240 min, blood samples were collected. HPLC-FLU was established to determine the plasma concentration of cirmarizine and the pharmacokinetic evaluation was carried out. RESULTS： The linear range of cinnarizine was 10-1 000 ng·mL^-1 （r=0.999 6） and the limit of detection of cinnarizine was 0.2 ng·mL^-1, the average recovery being 95.9% 98.6% with RSD〈8.6%. t1/2 was（87.73±22.54） min and AUC0-240min was （58 453±8 672,8）ng·min·mL^-1. CONCLUSION： This assay method is simple, precise and sensitive enough for the pharmacokinetic study of cinnarizine.