芒果核提取物动物体内外的抗菌作用及安全性研究

目的研究芒果核提取物动物体内外抑菌效果及其使用的安全性。方法采用二倍稀释法及琼脂扩散法，观察芒果核提取物对常见肠道感染致病菌（标准和耐药）志贺痢疾杆菌、福氏痢疾杆菌、大肠埃希菌的体外抑菌作用；采用小鼠复制感染模型观察芒果核提取物动物体内抑菌效果；用小鼠空腹经口灌胃给予芒果核提取物做急性毒性试验。结果芒果核提取物对标准和耐药志贺痢疾杆菌、福氏痢疾杆菌、大肠埃希菌有不同程度体外抑菌作用，其最低抑菌浓度（MIC）分别为7．8、7．8、7．8、15．6、15．6mg／ml和15．6mg／ml，抑菌圈直径分别为20、18、21、19、17、18mm；动物体内大、中、小剂量对（标准及耐药组）福氏痢疾杆菌及志贺痢疾杆菌感染小鼠有明显抑菌作用；小鼠灌胃最大耐受量40g／kg，相当临床用量的50倍。结论芒果核提取物对常见3种肠道感染菌具有较明显的体内外抑菌作用及具有良好的安全性。

Bacteriostasis and safty of mango seed extractive in vitro and in vivo

Objective To study the bacteriostasis and its practical safety of mango seed extractive in vivo and vitro. Methods The bacteriostasis in vitro of mango seed extractive, double-diluted and agar-diffused, on common intestinal infection pathogenic bacteria（standard and tolerated） ： Bacillus shigae, Shigella flexneri, Escherichia coli were observed; the bacteriostasis in vivo of mango seed extractive were observed on duplicated infected model mouse; the acute toxicity test was conducted in fasting mouse garaged with mango seed extractive. Results Mango seed extractive possesses bacteriostasis in vitro of different degrees on standard and tolerated Bacillus shigae, Shigella flexneri and Escherichia coli, respectively with minimal inhibitory concentrations 7.8 mg/ml, 7.8 mg/ml, 7. 8 mg/ ml, 15.6 mg/ml, 15.6 mg/ml, 15.6 mg/ml and with bacteriostasis collar diameters 20 ram, 18 mm, 21 mm, 19 mm, 17 mm, 18 mm. Large, medium and low dosages had evident bacteriostasis on the mice infected with Shigella flexneri and Bacillus shigae. Maximal tolerated dose of intragastric administration for the mouse was 40 g/kg, （ equivalently 50 times clinic dosage）. Conclusion Mango seed extractive possesses evident bacteriostasis and favorable safety in vivo and vitro on 3 types of common intestinal infection bacteria.