抑癌基因PTEN与Cyclin D1／CDK4在口腔鳞癌中的表达及其相关性分析

目的通过检测口腔鳞癌及癌前病变中抑癌基因VrEN与Cyclin D1／CDK4的表达，分析其相关性，初步探讨PTEN在口腔鳞癌中的意义。方法通过免疫组化sP法检测60例口腔鳞癌、15例单纯增生、25例异常增生中PTEN、Cyclin D1／CDK4的表达，以10例正常口腔黏膜为对照。结果在口腔鳞癌中PTEN阴性、弱阳性表达的占21．8％，显著低于正常组、单纯增生组及异常增生组（P〈0．01）；Cyclin D1及CDK4在OSCC中均以阳性表达及强阳性表达为主，分别占53．3％和100％，显著高于正常组、单纯增生组及异常增生组（P〈0．01）。PTEN与Cyclin D1、CDK4间呈显著的负相关关系，Cyclin D1与CDK4问呈显著的正相关关系。结论研究结果提示PTEN在口腔鳞癌的发生发展过程中起着一定的作用；在口腔鳞癌中PTEN可能是通过下调Cyclin D1／CDK4，从而抑制细胞的生长。

Expression of tumor suppressor gene PTEN,Cyclin DI,CDK4 in oral squamons cell carcinoma and the analysis of their correlations

Objective To detect the expression of PTEN, Cyclin D1 and CDK4 in oral squamous ceil carcinoma （OSCC） and analyze the relationship between PTEN, Cyclin D1 and CDK4. Methods Immunohistochemical staining by SP method was used to detect the expression of PTEN, Cyclin D1, CDK4 in 60 cases of OSCC, 25 cases of dysplasia, 15 cases of simple hyperplasia, and 10 cases of normal oral membrane. Results The negative or low expression of PTEN in OSCC group was 21.8%, which was significandy lower than that in normal, simple hyperplasia and dysplasia group. The positive expression of Cyclin D1 and CDK4 in most of OSCC group were observed, which was 53. 3% and 100%, respectively, and was much higher than those in normal, simple hyperplasia and dysplasia group. The negative correlation between PTEN with Cyclin D1 and CDK4 and the positive correlation between Cyclin D1 and CDK4 were observed. Conclusion Our findings suggest that the tumor suppress gene PTEN may play a role in the pathogenesis of OSCC. In OSCC, PTEN may down -regulate the expression of Cyclin D1/CDK4, which results in the suppression of cell growth.