自配试剂在Coulter流式细胞仪检测急性白血病免疫分型中的应用

目的分析自配Coulter流式细胞仪试剂在检测急性白血病免疫分型中的应用效果。方法将自配全血溶解试剂和鞘液及清洗液的理化性质、准确度和精密度以及对检测急性白血病免疫表型抗原的应用效果与原装试剂进行比较分析。结果自配试剂与原装试剂理化性质基本一致;两种试剂分别测定同一白血病免疫分型标本20次,结果各胞膜抗原批内CV值均〈2%;检测急性白血病免疫分型胞膜和胞浆多种抗原,分别与原装试剂比较,两者差异均无统计学意义（P〉0.05）,且检测胞浆抗原cMPO和cCD79a结果均呈高度正相关（r〉0.970）。结论自配Coulter流式细胞仪试剂对白血病免疫分型的检测效果好,可代替原装试剂进行应用并推广。

Application of Coulter flow cytometry with self-mix reagents for immunophenotyping of acute leukemia

Objective To analyze the effect of Coulter flow cytometry with self-mix reagents on immunophenotyping of acute leukemia. Methods The physical and chemical properties, accuracy, precision and the application effect on testing immunophenotyping of acute leukemia antigens between the original and the selfmix reagents of the hemolysis reagent, sheath fluid and cleaning fluid, were compared respectively. Results The physical and chemical properties were basically the same in two reagents; The within-run CV were less than 2% by testing several cell membrane antigens of acute leukemia with two reagents, every for 20 times; there were no statistics significance in detective results of membrance and cytoplasmic antigens between two reagents （ P 〉 0.05）, and the correlation coefficient of intracellular antigen cMPO and cCD79a were bigger than 0. 970. Conclasion The application effect of self-mix reagents is good, and the original reagents could be replaced by them when flow cytometry is applied to immunophenotyping of acute leukemia.