我国东南沿海地区副溶血弧菌O3:K6血清型耐药分析

目的 对分离自我国东南沿海地区的77株O3:K6血清型副溶血弧菌携带毒力基因情况及其耐药状况进行分析。方法 对2002-2016年分离自上海市、深圳市、江苏省、浙江省的77株O3:K6型副溶血弧菌tdh和trh基因进行PCR扩增检测，并用K-B纸片法检测菌株对15种抗生素的药敏情况。结果 77株O3:K6型副溶血弧菌tdh和trh基因的携带率分别为94.81%和1.30%。受试菌株对氨苄西林、链霉素、卡那霉素、磺胺异恶唑、环丙沙星的敏感率较低，分别为7.79%、18.18%、31.17%、49.35%、54.55%，其中对氨苄西林、磺胺异恶唑和链霉素的耐药率最高，分别为84.42%、36.36%、32.47%；对头孢曲松、头孢西丁、头孢吡肟、庆大霉素、阿奇霉素的敏感率较高，分别为96.10%、87.01%、81.82%、97.40%、93.51%；所有菌株对亚胺培南、复方新诺明、萘啶酸、多西环素、氯霉素均敏感。本研究中共分离到11株多药耐药菌株，全部为致病株，多药耐药率高达14.29%。结论 我国东南沿海地区分离的77株O3:K6型副溶血弧菌tdh携带率较高，trh携带率较低；对氨苄西林、磺胺异恶唑、氨基糖苷类、环丙沙星的敏感性较差，对其他临床常用抗生素比较敏感，同时存在多药耐药现象。

Antibiotic resistance of Vibrio parahaemolyticus serotype O3:K6 in southeastern coastal area of China

Objective To understand the virulence genes and antibiotic resistance of Vibrio parahaemolyticus serotype O3:K6 in southeastern coastal area of China. Methods Antibiotic susceptibility testing was performed for 77 strains of O3:K6 V. parahaemolyticus collected in Shanghai,Shenzhen,Zhejiang and Jiangsu provinces(municipality) during 2002-2016. Results Up to 94.81% and 1.30% of the tested strains were positive for tdh and trh respectively. The sensitive rates of the strains to some antibiotics were low,i. e. 7.79% to ampicillin,18.18% to streptomycin,31.17% to kanamycin,49.35% to sulfamethoxazole and 54.55% to ciprofloxacin,but the resistance rates were high to ampicillin(84.42%),sulfamethoxazole (36.36%) and streptomycin(32.47%). The sensitive rates to ceftriaxone(96.10%),cefoxitin(87.01%),cefepime(81.82%), gentamicin (97.40%) and azithromycin (93.51%) were high. All the strains were sensitive to imipenem,trimethoprim/sulfamethoxazole,nalidixic acid,doxycycline and chloramphenicol. Eleven strains(14.29%) were multidrug resistant,and all these strains were pathogenic. Conclusion Most O3:K6 V. parahaemolyticus isolated from southeastern coastal area of China were tdh positive and trh negative. Except ampicillin,sulfamethoxazole,aminoglycoside and ciprofloxacin,the O3:K6 V. parahaemolyticus isolates were sensitive to common used antibiotics,however,some strains showed multidrug resistance.