三七皂苷对人骨髓CD34+造血干/祖细胞的增殖分化作用

为了探讨三七皂苷 (PNS)对人骨髓CD34+ 造血干 /祖细胞的刺激增殖和诱导分化的作用 ,用DynalM 4 5 0CD34+ 免疫磁珠阳性选择法获取高纯度的人骨髓CD34+ 细胞 ,采用多向祖细胞 (CFU Mix)体外集落培养和流式细胞术检测细胞增殖与分化。结果显示 :经免疫磁珠阳性选择 ,从骨髓细胞收获的CD34+ 细胞获得率为 (1.0 3±0 .74 ) % ,流式细胞术分析纯度达到 86 % - 93%。PNS 10mg/L和 2 5mg/L能刺激CD34+ 细胞增殖 ,使CFU Mix集落生成增加 ,2 5mg/L的PNS对CFU Mix产率提高 (34.7± 16 .0 ) % (P <0 .0 1) ,是促进造血的最适浓度 ;PNS2 5 ,5 0和 10 0mg/L时 ,能诱导CD34+ 细胞向粒系细胞分化 ,粒系表面标记CD33+ 和CD15 + 的细胞百分比均明显高于无PNS的对照组 ,而红系细胞表面标记CD71+ 和G A+ 细胞百分比则无明显变化。结论 :PNS对CD34+ 造血干 /祖细胞不但具有显著的刺激增殖作用 ,而且能够诱导其向粒系细胞定向分化的效应

Proliferation and Differentiation of Human CD34+ Hematopoietic Stem/Progenitor Cells induced by Panax Notoginosides

The object of this study was to explore the effects of Panax notoginosides (PNS) on proliferation and differentiation of human CD34(+) stem/progenitor cells. CD34(+) cells were isolated from human bone marrow by using immune beads of Dynal M- 450 system. The cells were exposed to PNS at different concentrations in both liquid and semi-solid culture for 14 days. The cells were marked with monoclonal antibodies and analyzed by flow cytometry after culture. The CFU-Mix colony formation from CD34(+) cells was assayed. The results showed that: (1) The yield of CD34(+) cells after being selected by immune beads were (1.03 +/- 0.74)% out of bone marrow nuclear cells with purity of 86% - 93%. (2) PNS (10 - 25 mg/L) stimulated the proliferation of CD34(+) cells, and raised the colony numbers of CFU-Mix obviously in vitro. PNS 25 mg/L was the optimal concentration to promote proliferation of CD34(+) cells, the increasing rate of CFU-Mix colony was (34.7 +/- 16.0)%. (3) The differentiation of CD34(+) cells was induced by exposure to PNS (25, 50 and 100 mg/L) in liquid culture for 14 days. The percentages of CD33(+) and CD15(+) cells were increased after PNS exposure, which were significantly higher than those of control (P < 0.01), however CD71(+) and G-A(+) cells were no obviously difference after PNS treatment. In conclusion, Panax notoginosides not only promote the proliferation of CD34(+) cells, but also induce the differentiation committed to granulocytes.