原发性肝细胞癌组织中丙型肝炎病毒的检出及其相关意义的研究

目的　探讨丙型肝炎病毒 (HCV)感染导致肝细胞癌变的可能机理。方法　应用抗 - HCV NS3单克隆抗体和地高辛标记的 HCV全基因组 c DNA探针 ,对 19例伴单一 HCV感染的肝细胞癌石蜡组织切片进行了HCAg的免疫组化和原位杂交检测。结果　两种方法的阳性检出率分别为 89.5 % (17/19)和 5 7.9% (11/19)。HCAg主要分布于癌细胞和癌旁肝细胞的胞浆和胞核内 ,呈局灶或弥漫型分布 ,癌旁组织表达较癌巢显著 ,核型分布在癌旁组织中常见 ;HCV RNA仅见于癌或癌旁肝细胞的胞浆中 ,呈散在及局灶分布 ,弥漫型分布少见。结论　本研究结果支持 HCV感染可能导致原发性肝细胞癌的观点。 HCAg在癌旁肝细胞和癌细胞胞核中的存在提示其可能对肝细胞核产生基因调节作用 ,这是否是 HCV感染导致肝细胞癌变的可能机理之一 ,值得进一步研究阐明。

Detection of Hepatitis C Virus from Hepatocellular Carcinoma and Its Relationship to Hepatocarcinogenesis

Objective To get an insight of the relationship between hepatitis C Virus (HCV) infection and hepatocellular carcinoma (HCC). Methods A molecular pathology research was performed. Liver specimens from 19 HCC cases with HCV infection only were detected for HCV antigen (HCAg) and HCV RNA with monoclonal antibody (McAb) against HCV NS 3 antigen by means of immuno histochemistry staining and in situ hybridization using Dig labeled full length HCV cDNA Probe. Results In 17 of 19 (89.5%) cases, positive expression of the antigen was observed; 11 of 19 (57.9%) cases were positive for HCV RNA. The viral antigen was expressed in cytoplasm and nucleus of para cancerous hepatocytes and in cancer cells too, while the distribution of HCV RNA was only seen in cytoplasm of hepatocyte. Conclusion These results strongly support the relationship between HCV infection and the occurrence of HCC. The nucleic distribution of HCAg suggests a possible interaction between HCAg and the host genome, which may lead to hepatocarcinogenosis.