PCT、Fb和CRP检测在感染性疾病诊断中的应用

目的：探讨降钙素原（PCT）、纤维蛋白原（n）、C反应蛋白（CRP）检测在早期细菌性感染的诊断价值。方法：采用半定量的胶体金免疫结合法检测血清PCT，磁珠凝固法检测血浆Fb，免疫荧光比色法测定全血CRP水平。分别对细菌感染组91例，非细菌感染组108例，非感染组40例（对照组）进行PCT、Fb和CRP的测定。并同时检测白细胞计数和分类。结果：以血清PCT〉10．5ng／ml、Fb〉4．Og／L、CRP〉8．0mg／L为阳性阈值，细菌感染组PCT的阳性率为98．9％、浓度分别为（O．5～〈2．0）、（2．0～〈10）ng／ml、≥10ng／ml三个级别间；Fb的阳性率为93．4％，浓度为（6．19±1．44）g／L；CRP的阳性率为100％，浓度为（150．5±56．6）mg／L。非细菌感染组PCT的阳性率为18．5％，浓度为（0．5-〈2．0）ng／ml；Fb的阳性率为48．1％，浓度为（4．01±1．18）g／L；CRP的阳性率为47．2％，浓度为（48．9±5．61）mg／L。细菌感染组PCT阳性率明显高于非细菌感染组（P〈0．01）；n、CRP水平明显高于非细菌感染组（P〈0．01，P〈0．01）。非细菌感染组Fb、CRP水平明显高于非感染组（2．58±0．32）g／L（P〈0．01），Cae（14．5±0．3）mg／L（P〈0．01）。结论：PCT、Fb、CRP联检可作为早期细菌性感染的敏感诊断指标，指导临床合理用药和治疗。

Application of Procalcitonin,Fibrinogen,C-reactive Protein Detection in the Diagnosis of Infectious Diseases

Objective To study the value of procalcitonin （PCT）, fibrinogen （Fb）, C-reactive protein （CRP） detection in the early diagnosis of bacterial infection. Methods Semi-quantitative colloidal gold immuno-binding assay for the detection of PCT, magnetic coagulation assay for plasma Fb, immunofluorescence colorimetery of blood CRP level. Bacterial infection group 91 cases, a group of non-bacterial infection 108 eases, 40 cases of nonnfection group（control group） measured PCT, Fb and CRP levels respectively. Besides, also detecting white blood cell count and classification simueltaneously. Results serum PCT ≥ 0.5ng/ml, Fb 〉 4.0g/L, CRP 〉 8.0mg/L as the threshold of pesitivity, bacterial infection group the positive rate of PCT 98.9%, concentrations in 0.5- 〈 2.0,2.0 - 〈 10, I〉 10 ng/ml three levels respectively the positive rate of Fb 93.4%, concentration of（6.19 ＋ 1.44 ） g/L; CRP positive rate 100%, concentration of（ 150.5 ＋ 56.6） mg/L. Non bacterial infection group the positive rate of PCT 18.5%, at a concentration of 0.5 - 〈 2.0 ng/ml; the positive rate of Fb 48.1%, concentration of（ 4.01± 1.18） g/L; CRP positive rate was 47.2%, concentration of（48.9± 5.61 ） mg,/L. PCT positive rate of bacterial infection group was significantly higher than that of non-bacterialinfection group （P 〈 0. 01 ） ; Fb, CRP levels were remarkably higher than the non-bacterial infection group （ P 〈 0. 01, P 〈 0.01 ）. Non-bacterial infection group Fb, CRP levels expressed significantly higher than the non-infected greup（2.58 ＋0.32） g/L, CRP（ 14. 50 ＋ 0. 31 ） mg/L（P 〈 0. 01 ）. Conclusion PCT, Fb, CRP combined detection can be as early semsitive indicators of diagnosis for bacterial infections and to guide efficient treatment and drug use clinically.