细胞外基质蛋白1在肝癌中的表达及意义

目的：探讨细胞外基质蛋白1（extracellular matrix protein1，ECMl）在肝癌中的表达及意义。方法：应用免疫组织化学EnVision法，检测肝脏细胞株（正常肝细胞株LO2、肝癌细胞株HepG2）和肝脏组织（正常肝组织和肝癌组织）中ECM1的表达，并比较正常肝组织和肝癌组织之间的ECM1表达差异。结果：ECM1的表达如下：正常肝细胞株LO2（-），肝癌细胞株HepG2（＋）；正常肝组织阳性率20％（4／20），肝癌组织阳性率85．4％（70／82）。统计分析表明，对ECM1的表达，肝癌组织明显高于正常组织（P〈0．01）。结论：ECM1在肝癌组织中过表达。

Expression and Significance of Extracellular Matrix Protein 1 in Hepatocellular Carcinoma

To investigate the expression and significance of extracellular matrix protein 1 （ ECM1 ） in hepatocellular EnVision method was used to detect expression of ECM1 in liver cell strains （LO2, HepG2） and liver tissues （normal liver tissues and hepatocellular carcinoma tissues）, and the difference of ECM1 positive rates was compared between normal liver tissues and hepatocellular carcinoma tissues . Results： The expression of ECM1 were as follows： LO2 （ - ）, HepG2 （ ＋ ） ; the positive rate of normal liver tissues showed 20% （4/20）, hepatocellular carcinoma tissues presented 85.4% （70/82） ; the results showed that the expression of ECM1 in hepatocellular carcinoma tissues higher than that in normal liver tissues（P 〈0.01 ）. Conclusion ECM1 indicated over-expressed in hepatocellular carcinoma.