肾病综合征患儿输注悬浮红细胞前后血浆ET-1和血清MMP-2、MMP-9水平的变化及临床价值

目的：探讨肾病综合征患儿输注悬浮红细胞前后血浆ET-1和血清MMP-2、MMP-9水平的变化及临床意义。方法：采用放射免疫分析和酶联法对32例肾病综合征患儿进行了输注红细胞前后血浆ET-1和血清MMP-2、MMP-9测定，并与35名正常健康儿作比较。结果：肾病综合征患儿输注悬浮红细胞前血浆ET-1和血清MMP-2水平非常显著地高于正常儿组（P〈0．01），而血清MMP-9水平又显著地低于正常儿组（P〈0．01），经输注悬浮红细胞2周后与正常儿组比较仍有显著性差异（P〈0．05），且血清MMP-9水平与血浆ET-1和血清MMP-2水平呈现显著负相关（r=-0．4982、-0．014，P〈0．01）。结论：检测肾病综合征患儿输注悬浮红细胞前后血浆ET-1和血清MMP-2、MMP-9水平的变化对其病情发展及预后判定均有重要的临床价值。

Clinical Values of Measurement of Changes on Plasma ET-1 and Serum MMP-2, MMP-9 Levels After Transfusion of Red Blood Cells Suspension in Patients with Nephrotic Syndrome

Objective To explore the changes on plasma ET-1 and serum MMP-2, MMP-9 levels after transfusion of red blood cells suspension in patients with nephrotic syndrome. Methods Plasma ET-1（with RIA） ,serum MMP-2, MMP-9（ with ELISA） levels were determined in 32 patients with nephrotic syndrome and compared with 35 normal healthy children served as control. Result Before transfusion, plasma ET-1 and serum MMP-2 levels in the patients were significantly higher than those in controls（P 〈0.01 ）. But the MMP-9 level showed markedly lower（ P 〈0.01 ）. After a course for 2 weeks transfusion of red blood cells, the levels in patients though dropped notably but still remained higher than those in controls（P 〈0.05 ）. Conclusion Detection of plasma ET-1 and serum MMP-2, MMP-9 levels after tmmfusion RBCs suspension might be useful for monitoring progress and prognosis of patients with nephrotic syndrome and possess important clinical values .