| 人血管生成素-1真核表达载体的构建与表达 |
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| 引用本文： | 吕永强,童贻刚. 人血管生成素-1真核表达载体的构建与表达[J]. 临床军医杂志, 2013, 0(12): 1234-1236 |
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| 作者姓名： | 吕永强 童贻刚 |
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| 作者单位： | [1]解放军第210医院检验科,辽宁大连116021 [2]解放军军事医学科学院微生物流行病研究所,北京100071 |
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| 摘 要： | 目的构建人血管生成素-1的真核表达载体，真核表达人血管生成素-1蛋白并进行纯化。方法通过聚合酶链反应（PCR）将人血管生成素-1基因片段插入B7载体中构建真核表达载体，通过转染中国仓鼠卵巢（CHO）细胞进行真核表达。结果成功构建了B7Ang-1真核表达载体，在CHO细胞中进行了稳定表达，获得了纯化的人血管生成素-1蛋白。结论真核系统表达的人血管生成素-1蛋白具有良好的抗原性，为进一步的生物活性研究和临床应用奠定了基础。
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| 关 键 词： | 人血管生成素-1 聚合酶链反应 真核表达 |
Construction and expression of human angiopoietin-1 eukaryotic vector |
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| Lv Yongqiang,Tong Yigang. Construction and expression of human angiopoietin-1 eukaryotic vector[J]. Clinical Journal of Medical Officer, 2013, 0(12): 1234-1236 |
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| Authors: | Lv Yongqiang Tong Yigang |
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| Affiliation: | 2 ( 1. Department of Laboratory, PLA 210th Hospital, Dalian Liaoning 116021, China; 2. Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, PLA, Beijing 100071, China) |
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| Abstract: | Objective To construct the eukaryotic expression vector of human angiopoietin-1, and express and purify the human angiopoietin-1 protein. Methods The fragment of human angiopoietin-1 gene was inserted into B7 vector by polymerase chain reaction （PCR） and then transfected into Chinese hamster ovary （CHO） cells for eukaryotic expression. Results The B7Ang- 1 eukaryotic vector was constructed and stably expressed in CHO cell. The purified human angiopoietin-1 protein was obtained. Conclusion Human angiopoietin-1 protein is expressed in the eukaryotic system with good antigenicity. This study lays a good foundation for further study on the biological activity and clinical application of human angiopoietin-1. |
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| Keywords: | human angiopoietin-1 polymerase chain reaction eukaryotic expression |
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