胃腺癌组织Twist蛋白的表达及其与上皮-间质转化的关系

目的 分析胃腺癌组织中Twist蛋白的表达与临床病理参数之间的相关性及其与上皮-间质转化（EMT）的关系,探讨Twist的表达在判断患者预后中的意义.方法 采用免疫组织化学SABC法检测120例胃腺癌标本及其对应癌旁组织中Twist及EMT相关蛋白上皮钙黏素（E-cad）、神经钙黏素（N-cad）的表达情况,统计分析Twist表达与患者临床病理参数的关系及其与E-cad及N-cad表达的相关性.结果 在胃腺癌组织中Twist、E-cad、N-cad阳性率分别为59.1％（71/120）、36.6％（44/120）、47.5％（57/120）,癌旁组织中阳性率分别为17.5％（21/120）、93.3％（112/120）、11.6％（14/120）,两种组织间各指标阳性率差异均有统计学意义（均P＜0.05）.Twist的表达与肿瘤远处转移、淋巴结转移相关,且与浸润深度呈正相关（均P＜ 0.05）.Twist的表达与N-cad和E-cad的表达存在相关性（P＜0.05）.结论 胃腺癌组织中Twist、E-cad、N-cad的表达与癌旁组织明显不同,并且胃腺癌组织中Twist的表达与肿瘤的生物学特征密切相关;胃癌组织中Twist蛋白表达与EMT相关蛋白E-cad表达呈负相关,而与N-cad表达呈正相关.

Expression of Twist protein and its relationship with epithelial-mesenchymal transition phenomenon in gastric cancer

Objective To investigate the relationship between the expressions of Twist and clinical pathological parameters in gastric carcinoma,to explore its significance in judging the prognosis of patients,and to analyze the expression of Twist proteins in the epithelial-mesenchymal transition （EMT） phenomenon.Methods Immunohistochemistry was used to detect the expressions of Twist and EMT relative proteins E-cad,N-cad in 120 cases of gastric carcinoma specimens and corresponding adjacent normal tissue.Analysis The relationship between Twist expression and clinical pathological parameters,and the Twist expression and E-cad,N-cad were analyzed.Results In gastric carcinoma,Twist,E-cad,N-cad positive rates were 59.1％（71/120）,36.6 ％ （44/120） and 47.5 ％ （57/120）,and in adjacent tissues,the positive rate were 17.5 ％ （21/120）,93.3 ％ （112/120） and 11.6 ％ （14/120）.There were significant differences in each index between tumor tissues and adjacent tissues （P ＜ 0.05）.Patients with Twist positive expression showed higher incidence rates of lymph node metastasis （P ＜ 0.05） and distant metastasis （P ＜ 0.05） than those with Twist negative expression.The expression of Twist was significantly correlated in gastric carcinoma accompanied by low expression of E-cad and abnormal high N-cad expression （P ＜ 0.05）.Conclusions Compared with the adjacent tissue,the expression of Twist,E-cad and N-cad have significant differences,and the expression of Twist was closely related to the biological characteristics in gastric carcinoma.The expression of Twist in gastric cancer have negative correlation with the EMT relative factor E-cad,while positive correlation with the N-cad.