On the metabolism of allopurinol. Formation of allopurinol-1-riboside in purine nucleoside phosphorylase deficiency |
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Authors: | S Reiter H A Simmonds D R Webster A R Watson |
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Affiliation: | 1. Purine Laboratory, Guy''s Hospital Medical School, London, U.K.;2. Royal Manchester Children''s Hospital (A.R.W.), Manchester, U.K. |
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Abstract: | Allopurinol-1-riboside, a major metabolite of allopurinol, is commonly thought to be directly synthesized by purine nucleoside phosphorylase (PNP) in vivo. As this enzyme is otherwise believed to function in vivo primarily in the direction of nucleoside breakdown, we have determined by high performance liquid chromatography and a conventional chromatographic method the urinary metabolites of allopurinol in a child deficient of PNP. In this patient approximately 40% of urinary allopurinol metabolites consisted of allopurinol-1-riboside, thus proving the possibility of indirect formation of allopurinol-1-riboside via allopurinol-1-ribotide in vivo, catalysed by hypoxanthine guanine phosphoribosyltransferase (HGPRT) and a phosphatase. |
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Keywords: | Present address of the author to whom correspondence should be sent: Dr. S. Reiter Medizinische Poliklinik der Universität Pettenkoferstr 8a 8000 München 2 Federal Republic of Germany. |
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