胃癌组织中T细胞及其亚群ICOS分子的表达及其意义

目的探讨胃癌组织中T细胞共刺激分子ICOS及其亚群的表达及意义。方法应用流式细胞术检测38例胃癌和21例健康人（对照组）外周血T细胞亚群及其共刺激分子ICOS的表达。结果胃癌组与对照组比较：CD3^＋T细胞表达（53．61±13．84）／（72．07±7．83）％，P〈0．01；CD3^＋CD4^＋T细胞表达（29．84±9．71）／（38．79±5．08）％。P〈0．01；CD3^＋ICOS^＋T细胞表达（25．80±10．56）／（0．82±0．98）％，P〈0．01；CD3^＋CD8^＋ICOS^＋T细胞表达（1．57±1．99）／（0．02±0．04）％，P〈0．01；CD3^＋CD8^＋ICOS^-T细胞表达（16．06±6．94）／（20．56±6．54）％，P〈0．05。胃癌组患者手术前和手术后1周外周血T细胞亚群的差异无统计学意义（P〉0．05）。结论胃癌患者T细胞数量明显减少，T细胞共刺激分子ICOS表达增高，CD4^＋T细胞显著减少。

Expression of Inducible Costimulator and Variance of T Cell Subpopulations in Patients with Gastric Cancer

Objective To investigate the role of expression of T cell costimulatory molecule ICOS and variance of T cell subpopulations in the development and prognosis of gastric cancer. Methods The peripheral blood lymphocytes were tested for T cell subpopulations and T cell costimulatory molecule ICOS by flow cytometry in 38 patients with gastric cancer and 21 healthy peoples as control group. Results The expressions of T cell costimulatory molecule ICOS in the gastric cancer and healthy controls were （25.80 ± 10.56） % and （0.82 ±0.98） % （P 〈0.01 ） ; expression percentage of total T cell （ CD3^＋ ） were （53.61 ±13.84）vs（72.07 ±7.83）% ,P 〈0.01 ; expression percentage of CD4^＋T cell （CD4^＋ CD3^＋） were （29.84 ±9.71）vs （38.79 ± 5.08 ） %, P 〈 0.01 ; expression percentage of CTL cell （ CD8^＋ ICOS ^＋ CD3 ^＋ ） were （ 1.57 ± 1.99 ） vs （ 0.02 ± 0.04 ） %, P 〈 0.01 ; expression percentage of CD8^ ＋ inhibitory T cell （ CD8^＋ICOS ^- CD3 ^＋ ） ratio were （ 16.06 ± 6.94） vs （ 20.56 ± 6.54 ） %, P 〈 0.05. No difference of expression was found in peripheral T cell subpopulations of pestoperativc patients with gastric cancer after one week （ P 〉 0.05 ）. Conclusion T cell number is fall, and T cell eostimulatory molecule ICOS useless expression is increase in patients with gastric cancer. CD4^＋ ＋T cell subpopulation is significantly decreased in patients with gastric cancer.