纳洛酮对缺血／再灌注损伤心肌炎症介质生成的影响

目的本研究通过家兔心肌缺血再灌注动物模型观察纳洛酮对心肌缺血／再灌注损伤中白细胞介素-8（IL-8）及血栓素B2／6-酮-前列腺素F1α变化的影响。方法30只新西兰大白兔随机分为再灌注组（10只），结扎冠状动脉左前降支1h／开放4．5h；纳洛酮组（10只），于结扎同时静脉注射纳洛酮0．4mg·kg^-1；假手术组（10只）。测缺血／再灌注组和治疗组再灌注4．5h缺血区心肌IL-8含量及各组动脉结扎前及再灌注4．5h后的血清IL-8含量及血栓素B2、6-酮．前列腺素F1α含量。结果再灌注后，再灌注组缺血区心肌组织及血清IL-8含量明显升高，显著高于纳洛酮组和假手术组（P〈0．01）；再灌注组血浆血栓素B2含量及血栓素B2／6-酮-前列腺素F1α比值显著高于纳洛酮组和假手术组（P〈0．01）。结论纳洛酮可以抑制家兔心肌缺血／再灌注后炎症介质IL-8及血栓素B2的升高。

Naloxone Inhibits Generation of IL-8 and TXA2 from Ischemic and Reperfused Myocardium

Aim To study the effect of Naloxone on inflammatory factor interleukin-8 （IL-8） ,TXA2 and 6-Keto-PGF1α generated from ischemic and reperfused myocardium. Method 30 mature male anesthetized New Zealand white rabbits were divided into three groups randomly： sham-operation group（ n = 10）, reperfusion group（ n = 10） and therapy group （ischemia/reperfusion combined with Naloxone intravenous therapy, n = 10）. Coronary Left anterior descending artery was occluded for 1 hour and reperfused for 4. 5 hours. IL-8 from blood serum and ischemic tissue, TXB2 and 6-Keto- PGF1α from blood serum were detected using radioimmunoassays. Result 1. After reperfusion,the serum concentration of IL-8 of ireperfusion group was much higher than that of the sham-operation group. The concentration of IL-8（whatever of tissue or serum） of reperfusion group was obviously higher than that of the therapy group（ P 〈0.01 ） 2 The concentration of TXB2 and ratio of TXB2/6-Keto-PGF1α of the reperfusion group were much higher than those of the therapy group and sham-operation group（ P 〈0. 01 ）. There was no significant difference in the concentration of 6-Keto-PGF1α between the three groups. Conclusion Ischemia and reperfusion leads to the generation and discharge of IL-8 and TXA2 from injured myocardium; Naloxone may significantly inhibit these inflammatory reactions.