釉基质蛋白对碱热处理纯钛表面磷灰石生成的影响

目的：研究釉基质蛋（EMPs）对碱热处理纯钛表面磷灰石涂层生成的影响。方法：采用乙酸法提取猪EMPs。纯钛片碱热处理后，在含不同浓度EMPs（0、50、100、150、200μg／mL）的模拟体液中浸泡1周、2周后取出。扫描电镜观察表面形貌，x射线衍射分析晶相结构。结果：各组试件表面均有类骨磷灰石（CHA）生成。EMPs浓度为50、100μg／mL时，钙磷涂层的沉积受到抑制；EMPs浓度为150、200μg／mL时，钙磷涂层的沉积增强，且涂层粗糙度增加，有直径约300～600nm孔隙生成，晶体呈（002）方向择优取向生长。结论：EMPs对碱热处理纯钛表面磷灰石生成的影响与浓度密切相关，较高浓度EMPs可促进钛表面生成CHA，并改变磷灰石晶体的形态与生长方向。

Effects of Enamel Matrix Proteins on the Growth of Apatite Layer on Alkali Heat-treated Titanium Surface.

Objective： To evaluate the effects of enamel matrix proteins （EMPs） on the growth of apatite layers on alkali heat--treated titanium surface. Methods： EMPs were extracted from porcine tooth germs by the use of acetic acid. Pure titanium samples with alkali heat--treatment were soaked in a blank or EMPs containing （50,100,150, 2008g/mL） stimulated body fluid （SBF） at 32℃ for 1 or 2 weeks respectively. The morphologies of apatite layers were observed with scanning electron microscope （SEM）. X--ray diffraction （XRD） was used to identify the crystal structure of the surface layers. Results： Apatite layers were found on titanium surfaces of all groups, which were proved as carbonate hydroxyapatite （CHA）. EMPs, at the concentrations of 50, 100μg/mL, inhibited the growth of apatite crystals. Nevertheless, EMPs, at high concentrations （150, 200μg/mL）, could promote apatite deposi tion with a preferential orientation of （002） plane and enhance the roughness of apatite layers with many pores （300 --600nm）. Conclusion： EMPs, at high concentrations, promotes apatite deposition on alkali heat treated titanium surface, and affects the growth and orientation of the apatite crystals, depending on the dose.