| PFGE法分析多血清型副溶血性弧菌引起的食物中毒 |
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| 引用本文： | 孙永祥,丁水军,罗芸.PFGE法分析多血清型副溶血性弧菌引起的食物中毒[J].中国卫生检验杂志,2011(10):2444-2445. |
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| 作者姓名： | 孙永祥 丁水军 罗芸 |
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| 作者单位： | 浙江省杭州市萧山区疾病预防控制中心;浙江省疾病预防控制中心; |
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| 摘 要： | 目的:对从食物中毒样品中分离到的7株副溶血性弧菌作常规鉴定及脉冲场凝胶电泳(PFGE)同源性分析,以明确致病因子。方法:按GB/T4789.7-2008进行副溶血性弧菌分离、生化鉴定、神奈川试验、血清分型;Pulse-Net PFGE分子分型标准实验室操作方法作同源性分析。结果:7株副溶血性弧菌分成三个血清型,即O3∶K6型5株,O4∶K8型和O1∶K56型各1株,分别属于三种不同的PFGE条带型,其中6株菌神奈川试验阳性。结论:这次食物中毒可能为以O3∶K6型为主的不同克隆群副溶血性弧菌混合感染所致。
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| 关 键 词： | PFGE分型 副溶血性弧菌 食物中毒 多个血清型 |
Analysis on outbreak of food poisoning induced by Multi-serotype Vibrio parahaemolyticus with pulsed-field gel electrophoresis typing |
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| SUN Yong-xiang,DING Shui-jun,LUO Yun.Analysis on outbreak of food poisoning induced by Multi-serotype Vibrio parahaemolyticus with pulsed-field gel electrophoresis typing[J].Chinses Journal of Health Laboratory Technology,2011(10):2444-2445. |
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| Authors: | SUN Yong-xiang DING Shui-jun LUO Yun |
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| Institution: | SUN Yong-xiang1,DING Shui-jun1,LUO Yun2 (1.Xiaoshan District Center for Disease Control and Prevention,Hangzhou 311201,China,2.Zhejiang Provincial Center for Disease Control and Prevention,Hangzhou 3100051,China) |
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| Abstract: | Objective:To study the molecular epidemiology and homology relationship among 7 strains of Vibrio parahaemolyticus isolated from patients samples during food poisoning outbreak with routine experiment and pulsed-field gel electrophoresis(PFGE)typing,to trace the source of infections so as to support the ascertainment of epidemic control strategy.Methods: Strains isolated from samples as mentioned above were determined according to the method of GB/T 4789.7-2008.Biochemical identification,Kanagawa test,serol... |
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| Keywords: | Pulsed-field gel electrophoresis(PFGE) typing Vibrio parahaemolyticus Food poisoning Multi-serotype |
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