| 台州市乙型流感病毒分离、鉴定及HA1基因特性研究 |
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| 引用本文： | 李桂霞,郑官增,裘丹红,海岗,沈伟伟,章敏杰.台州市乙型流感病毒分离、鉴定及HA1基因特性研究[J].中国卫生检验杂志,2011(9). |
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| 作者姓名： | 李桂霞 郑官增 裘丹红 海岗 沈伟伟 章敏杰 |
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| 作者单位： | 浙江省台州市疾病预防控制中心;浙江省海正药业; |
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| 基金项目： | 台州市科技局社会发展类重点项目(100KY60) |
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| 摘 要： | 目的:了解台州市Yamagata系乙型流感病毒的基因特性、变异情况和WHO推荐流感疫苗株对人体的保护情况。方法:用狗肾传代细胞(MDCK)分离流感病毒,用标准血清及荧光定量RT-PCR方法鉴定。选2011年分离株进行HA1基因序列测定,用DNAman和DNAstar生物学软件进行序列分析。结果:2011年分离株HA1基因与参考毒株核苷酸同源性为88.6%～96.9%,氨基酸同源性为89.2%～98.0%,存在8个潜在的糖基化位点,比同谱系疫苗株在197位增加了一个糖基化位点NKT。与Yamagata系代表株在进化树的同一支上,与Victoria系代表株比,氨基酸162位缺失K。结论:本地2011年分离株与近年来WHO推荐的流感疫苗株在HA1片段有明显的氨基酸变异。2010年-2011年台州市主要分离到Yamagata系乙型流感病毒,而WHO推荐使用的2010年-2011年乙型流感疫苗株B/Brisbane/60/2008属于Victoria系,因此本年度流感疫苗对我市乙型流感不能提供最佳的保护性。
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| 关 键 词： | 乙型流感病毒 Yamagata系 HA1基因 基因特性 |
Phylogenetic analysis of HA1 gene of influenza B viruses isolated and identificated in TaiZhou city |
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| LI Gui-xia,ZHENG Guan-zeng,QIU Dan-hong,HAI Gang,SHEN Wei-wei,ZHANG Min-jie.Phylogenetic analysis of HA1 gene of influenza B viruses isolated and identificated in TaiZhou city[J].Chinses Journal of Health Laboratory Technology,2011(9). |
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| Authors: | LI Gui-xia ZHENG Guan-zeng QIU Dan-hong HAI Gang SHEN Wei-wei ZHANG Min-jie |
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| Institution: | LI Gui-xia1,ZHENG Guan-zeng1,QIU Dan-hong1,HAI Gang2,SHEN Wei-wei1,ZHANG Min-jie1(1.Taizhou Center for Disease Control and Prevention,Taizhou 318000 China,2. Zhejiang Hisun Pharmaceutical Co.Ltd.Taizhou 318000,China) |
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| Abstract: | Objective: To analyze the genetic characteristics and variation influenza B virus isolated in Taizhou and evaluate the protective effect of the vaccine strains recommended by WHO.Methods: Influenza viruses were isolated in MDCK cell and identified by standard serum and real-time RT-PCR. Isolates in 2011 were selected to conduct HA1 gene sequencing,DNAman and DNAstar were used to conduct sequence analysis.Results: The homology of nucleotide acids was 88.6%~96.9% between the isolated virus strains and referen... |
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| Keywords: | Influenza viruses B Yamagata-like HA1 gene Genetic characteristics |
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