| 福州市麻疹病毒分离株基因特性研究 |
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| 引用本文： | 姚栩,杨秀惠,陈智伟,郭燕,黄晓霞,张宏,陈艳. 福州市麻疹病毒分离株基因特性研究[J]. 中国卫生检验杂志, 2011, 0(9) |
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| 作者姓名： | 姚栩 杨秀惠 陈智伟 郭燕 黄晓霞 张宏 陈艳 |
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| 作者单位： | 福州市疾病预防控制中心;福建省疾病预防控制中心; |
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| 基金项目： | 福州市科技局;榕科[2009]109号(2009-S-93) |
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| 摘 要： | 目的:探究福州市麻疹病毒基因的遗传变异特点及种系进化分布,为控制和消除麻疹提供科学依据。方法:应用Vero/slam细胞进行麻疹病毒分离,用RT-PCR方法扩增分离株病毒的N基因C末端450个核苷酸片段,扩增产物经纯化后,进行N基因核苷酸序列测定并推导出其aa序列,然后用DNASTAR MegAlign序列分析软件进行基因种系进化特性分析。结果:福州市麻疹分离株均为H1a基因亚型,3株病毒核苷酸同源性为99.3%～100%,在遗传树图中分属于1个独立分支,与疫苗株S191相比核苷酸同源性为87.5%,aa同源性为91.9%～92.1%。结论:福州麻疹野病毒与疫苗株S191在核苷酸与aa序列均存在较大差异,在麻疹监测工作中应进一步加强麻疹病原学的监测和麻疹遗传变异特性的研究。
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| 关 键 词： | 麻疹病毒 基因特性 |
Research on genetic characteristics of measles virus isolated in Fuzhou |
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| YAO Xu,YANG Xiu-hui,CHEN Zhi-wei,GUO Yan,HUAN Xiao-xia,ZHANG Hong,CHEN Yan. Research on genetic characteristics of measles virus isolated in Fuzhou[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(9) |
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| Authors: | YAO Xu YANG Xiu-hui CHEN Zhi-wei GUO Yan HUAN Xiao-xia ZHANG Hong CHEN Yan |
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| Affiliation: | YAO Xu1,YANG Xiu-hui2,CHEN Zhi-wei1,GUO Yan1,HUAN Xiao-xia1,ZHANG Hong1,CHEN Yan1 (1.Fuzhou Center for Disease Control and Prevention,Fuzhou 350005,China,2.Fujian Center for Disease Control and Prevention,Fuzhou 350001,China) |
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| Abstract: | Objective: To understand the genetic variation characteristics of measles viruses and evolutionary distribution.Methods: Measles viruses were isolated with Vero/slam cell.The 450 C-terminal nucleotide fragment of the nucleoprotein(N) gene was amplified by RT-PCR and PCR products were purified and conducted determination of nucleotide sequences of N gene to deduce the amino acid sequences via bio-informatics.Finally the phylogenetic tree was analyzed with DNASTAR MegAlign soft ware.Results: The Measles virus... |
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| Keywords: | Measles Virus(MV) Genetic characteristics |
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