| 女性维生素D受体基因FokI遗传多态性对维生素D受体蛋白的影响 |
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| 引用本文： | 黄友泽,白永恒,陆红,曾云祥,陈以勒,潘贻飞. 女性维生素D受体基因FokI遗传多态性对维生素D受体蛋白的影响[J]. 中国卫生检验杂志, 2011, 0(11): 2657-2658,2661 |
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| 作者姓名： | 黄友泽 白永恒 陆红 曾云祥 陈以勒 潘贻飞 |
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| 作者单位： | 温州市第八人民医院;温州医学院附属第一医院; |
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| 基金项目： | 温州市鹿城区科技计划项目(S10106) |
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| 摘 要： | 目的:探讨女性人群维生素D受体(VDR)基因5’端转录起始位点FokI多态性对VDR蛋白水平的影响。方法:以229例女性人群作为研究对象,采用PCR-RFLP检测其VDR基因FokI位点多态性,ELISA检测血浆VDR蛋白水平。结果:FokI位点基因型符合H-W平衡检验;基因型以杂合子Ff为主,占50.26%;FF基因型女性的血浆VDR水平为82.67±34.14 pmol/ml,显著低于Ff(103.78±17.48 pmol/ml)或ff基因型(114.73±53.00 pmol/ml);VDR水平与年龄明显相关。结论:女性人群VDR基因FokI多态性与血浆VDR蛋白水平相关,FF基因型具有较低的VDR水平。
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| 关 键 词： | 维生素D受体 基因型 PCR-RFLP ELISA |
Effect of vitamin D receptor(VDR) FokI polymorphism on VDR protein levels in females |
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| HUANG You-ze,BAI Yong-heng,LU Hong,ZENG Yun-xiang,CHEN Yi-le,PAN Yi-fei. Effect of vitamin D receptor(VDR) FokI polymorphism on VDR protein levels in females[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(11): 2657-2658,2661 |
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| Authors: | HUANG You-ze BAI Yong-heng LU Hong ZENG Yun-xiang CHEN Yi-le PAN Yi-fei |
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| Affiliation: | HUANG You-ze1,BAI Yong-heng2*,LU Hong2,ZENG Yun-xiang1,CHEN Yi-le1,PAN Yi-fei2 |
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| Abstract: | Objective:To explore the effect of FokI polymorphisms of the VDR gene on VDR protein levels in females.Methods: A total of 229 females were enrolled in our study,and the genotypes were detected by Polymerase chain reaction-restrained fragment length polymorphism technique(PCR-RFLP).The levels of VDR protein were measured by enzyme-linked immunosorbent assay(ELISA).Results: The frequency of FokI genotype followed the Hardy-Weinberg equilibrium test.The distribution of FokI Ff genotype is most,and the frequen... |
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| Keywords: | Vitamin D receptor Genotype PCR-RFLP ELISA |
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