| 高效液相色谱荧光检测法测定辣椒油、辣椒粉、火锅底料中的罗丹明B染料 |
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| 引用本文： | 汤建春,龙朝阳,许秀敏,吴瑞英,孟宇航. 高效液相色谱荧光检测法测定辣椒油、辣椒粉、火锅底料中的罗丹明B染料[J]. 中国卫生检验杂志, 2011, 0(12) |
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| 作者姓名： | 汤建春 龙朝阳 许秀敏 吴瑞英 孟宇航 |
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| 作者单位： | 广东省江门市病症预防控制中心;广东省疾病预防控制中心; |
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| 摘 要： | 目的:建立高效液相色谱荧光检测法测定辣椒油、辣椒粉、火锅底料中的罗丹明B染料的分析方法。方法:样品经30%甲醇水溶液提取后,采用C18反相色谱柱分离,以甲醇和水为流动相进行梯度洗脱,荧光检测器测定,Ex:550 nm,Em:580 nm。结果:罗丹明B染料在2.0 ng/ml～200 ng/ml质量浓度范围内线性关系良好,相关系数r2为0.9998,方法检出限为0.02 mg/kg,方法回收率为83%～102%,精密度(RSD)1.3%～3.2%。结论:该方法简便、快速、灵敏度高、回收率高,适用于辣椒粉和辣椒油和火锅底料中罗丹明B染料的测定。
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| 关 键 词： | 高效液相色谱 荧光检测器 罗丹明B |
Determination of rhodamine B in chili powder,chili oil and hotpot additive by high performance liquid chromatography with fluorescence detector |
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| TANG Jian-chun,LONG Chao-yang,XU Xiu-min,WU Rui-ying,MENG Yu-hang. Determination of rhodamine B in chili powder,chili oil and hotpot additive by high performance liquid chromatography with fluorescence detector[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(12) |
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| Authors: | TANG Jian-chun LONG Chao-yang XU Xiu-min WU Rui-ying MENG Yu-hang |
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| Affiliation: | TANG Jian-chun1,LONG Chao-yang2,XU Xiu-min2,WU Rui-ying1,MENG Yu-hang1(1.Jiangmen Center for Disease Control and Prevention,Jiangmen 529030,China,2.Center for Disease Control and Prevention of Guangdong Province,Guangzhou 510300,China) |
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| Abstract: | Objective:A method was developed for determination of rhodamine B in chili powder,chili oil and hotpot additive by high performance liquid chromatography with fluorescence detector.Methods: The samples were extracted with 30% methanol-water,the target analytes were separated on C18 reversed-phase column,detected by fluorescence detector with gradient elution using methanol-water as mobile phases,Ex:550 nm,Em:580 nm.Results: The linear range was 2.0 ng/ml~200 ng/ml,r2=0.9998,the detection limit was 0.02 mg/k... |
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| Keywords: | High performance liquid chromatography Fluorescence detector Rhodamine B |
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