| 导流杂交基因芯片检测HPV16、18亚型的准确度分析 |
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| 引用本文： | 陈彤岩,刘志忠,杨军,叶虹.导流杂交基因芯片检测HPV16、18亚型的准确度分析[J].中国卫生检验杂志,2011(12). |
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| 作者姓名： | 陈彤岩 刘志忠 杨军 叶虹 |
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| 作者单位： | 首都医科大学附属北京天坛医院检验科; |
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| 摘 要： | 目的:验证凯普HPV分型检测系统检测HPV16、18型别结果的准确性,分析判读差异的原因。方法:选取110例凯普HPV16、18分型检测阳性的标本,根据显色程度分为显色正常82例(阳性),显色弱28例(弱阳性21例、极弱7例)。使用型别特异性引物PCR扩增+测序方法进行对照验证,判断其检测的准确度。结果:总共110例样本中,有6例显色弱的标本检测阴性,与标准方法比较,基因芯片结果总符合率94.5%,二者差异没有统计学意义(P>0.05)。不同程度显色标本与标准方法比较,显色正常的82例标本全部检出,符合率100%,21例弱阳性标本,4例未检出,符合率81.0%,7例极弱阳性标本,2例未检出,符合率71.4%。显色弱的样本检测符合率显著低于显色正常标本,χ2=11.9,P<0.01。结论:凯普基因芯片法检测HPV16、18亚型具有较高的准确度,但芯片上相应着色点显色弱,可能导致检测结果错误。
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| 关 键 词： | 乳头状瘤病毒 人 基因芯片 基因型 |
Analysis of the accuracy of flow-through hybridization and gene chip in human papillomavirus 16/18 subtype detection in clinic |
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| CHEN Tong-yan,LIU Zhi-zhong,YANG Jun,YE Hong.Analysis of the accuracy of flow-through hybridization and gene chip in human papillomavirus 16/18 subtype detection in clinic[J].Chinses Journal of Health Laboratory Technology,2011(12). |
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| Authors: | CHEN Tong-yan LIU Zhi-zhong YANG Jun YE Hong |
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| Institution: | CHEN Tong-yan,LIU Zhi-zhong,YANG Jun,YE Hong(Department of Clinical Laboratory,Beijing Tiantan Hospital affiliated to Capital Medical University,Beijing 100050,China) |
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| Abstract: | Objective:To evaluate the accuracy of flow-through hybridization and gene chip on human papillomavirus 16/18 subtype detection,and find out the reason for misinterpretation.Methods: The study involved 110 women who were infected by high-risk human papillomavirus(HPV) subtypes(including HPV16 or/and 18).According to the color test,all the patients were divided into normal(positive,n=82) and weak color test(weak positive,n=21;extremely weak,n=7) groups.The results of the gene chip were compared with those of ... |
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| Keywords: | Papillomavirus human Gene chip Genotype |
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