| 荧光PCR法与细菌培养法在食源性致病检测中的应用 |
| |
| 引用本文： | 王蒋丽,李彩云,曾强,王伟,何旭鑫,高杰,王辉,李洁. 荧光PCR法与细菌培养法在食源性致病检测中的应用[J]. 中国卫生检验杂志, 2011, 0(10) |
| |
| 作者姓名： | 王蒋丽 李彩云 曾强 王伟 何旭鑫 高杰 王辉 李洁 |
| |
| 作者单位： | 河北省承德市疾病预防控制中心;河北省承德县妇幼保健院;河北省宽城县中医院; |
| |
| 摘 要： | 目的:通过荧光PCR法与细菌培养法在食源性致病菌检测中的应用,比较两种方法的敏感性、特异性、时限性、重复性。方法:2009年1月-2010年12月采集的样品进行常见食源性致病的细菌培养,随机抽取一部分样品进行荧光PCR检测。结果:细菌培养法在257份样品中检出单核细胞增生李斯特菌5株、金黄色葡萄球菌5株,检出率3.9%。所检出致病菌经荧光PCR法符合结果,符合率100%。随机抽取各类产品的20%共计49份样品进行沙门菌、大肠埃希菌O157:H7、单核细胞增生李斯特菌、副溶血弧菌、金黄色葡萄球菌等核酸试剂盒检测。结果检出沙门菌1份、金黄色葡萄球菌2份、单核细胞增生李斯特菌1份、副溶血弧菌1份,其余致病菌核酸检测均未检出,检出率10%。结论:与传统的细菌培养法相比,荧光PCR法可提高食源性致病菌的检出率、缩短检出时间、适用于食源性致病菌的检测。
|
| 关 键 词： | 食源性致病菌 荧光定量PCR 细菌培养 检验 |
Application of FQ-PCR and bacterial culture in food-borne pathogens detection |
| |
| WANG Jiang-li,LI Cai-yun,ZENG Qiang,WANG Wei,HE Xu-xin,GAO Jie,WANG Hui,LI Jie. Application of FQ-PCR and bacterial culture in food-borne pathogens detection[J]. Chinses Journal of Health Laboratory Technology, 2011, 0(10) |
| |
| Authors: | WANG Jiang-li LI Cai-yun ZENG Qiang WANG Wei HE Xu-xin GAO Jie WANG Hui LI Jie |
| |
| Affiliation: | WANG Jiang-li1,LI Cai-yun2,ZENG Qiang1,WANG Wei1,HE Xu-xin1,GAO Jie1,WANG Hui2,LI Jie3(1.Chengde Center of Disease Prevention and Control,Chengde 067000,China,2.Chengde County Maternal and Child Health Hospital,Chengde County 067400,3.Kuancheng County Traditional Chinese Medicine Hospital,Kuancheng County,067411,China) |
| |
| Abstract: | Objective:Sensitivity,specificity,timeliness and repeatability of FQ-PCR and bacterial culture were compared in the detection of food-borne pathogens.Methods: Common food-borne pathogenic bacterial culture was done by using samples collected from Jan 2009 to Dec 2010,and then some samples were selected randomly to make FQ-PCR detection.Results: 5 Listeria monocytogenes bacteria and 5 Staphylococcus aureus are detected by using bacterial culture among 257 samples,with a detection rate of 3.9%.The pathogens a... |
| |
| Keywords: | Food-borne pathogens FQ-PCR Bacterial Culture Detection |
| 本文献已被 CNKI 等数据库收录！ |
|
