蚕丝-PLGA混合编织支架材料的细胞毒性检测

目的:对蚕丝-PLGA细丝混合编织支架体外进行细胞毒性实验,探讨蚕丝-PLGA混合编织支架材料的细胞毒性作用情况,为该材料临床应用提供理论依据。方法:制备蚕丝-PLGA细丝混合编织支架,并制备该支架材料的浸提液。通过MTT法检测25%、50%、100%浓度的蚕丝-PLGA混合编织支架材料浸提液对兔骨髓间充质干细胞(MSCs)的细胞毒性作用,以第1,3,5,7天为检测时间点,观察细胞生长状况,计算细胞相对增殖率,并对材料的细胞毒性进行分级。以正常培养基为阴性对照组,0.64%苯酚溶液为阳性对照组。结果:MSCs在不同浓度实验组及阴性对照组中生长均好,细胞呈长梭形等形态,细胞形态饱满有光泽,MTT法结果表明各时间点各浓度实验组与阴性对照组之间的A值差异不显著(P>0.05),各时间点各浓度浸提液对MSCs细胞的相对增殖率均在93%以上,毒性分级为0级或1级,无细胞毒性。结论:蚕丝-PLGA混合编织支架材料细胞相容性良好,无细胞毒性,符合材料毒性的安全标准。

Cytotoxicity test of silk-PLGA scaffold material on MSCs in vitro

Objective:To investigate cytotoxicity of silk fibroin fiber-PLGA filament blend braided scaffold material in vitro test,and provide theoretical references for clinical practice of this material.Methods: Silkworm silk fibroin fiber was mixed with PLGA filament at ratio of 2:3,then silk-PLGA scaffold was prepared by braiding them.Cell observation method and MTT assay were used to detect the cytotoxicity of silk-PLGA braided scaffold with extraction solutions.The cytotoxicity of the scaffold extract at concent...