| 不同树突状细胞对CD4~+CD25~+Foxp3~+调节性T细胞体外扩增的影响 |
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| 引用本文： | 丰涛,杨洁,谢如锋,刘嬿,任亚娜,范华骅.不同树突状细胞对CD4~+CD25~+Foxp3~+调节性T细胞体外扩增的影响[J].中国输血杂志,2009,22(4). |
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| 作者姓名： | 丰涛 杨洁 谢如锋 刘嬿 任亚娜 范华骅 |
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| 作者单位： | 1. 华东师范大学,生命科学学院,生物医学系,上海200062;上海市血液中心,血液工程研究室
2. 上海市血液中心,血液工程研究室 |
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| 基金项目： | 上海市科学技术委员会基金资助(编号:05XD14029) |
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| 摘 要： | 目的利用不同种类树突状细胞(dendritic cells,DC)体外扩增获得表型和功能稳定的CD4+CD25+Foxp3+调节性T细胞(Treg)。方法免疫磁珠法(MACS)分离Balb/c小鼠CD4+CD25+调节性T细胞,利用与调节性T细胞同基因或异基因成熟DC、未成熟DC和调节性DC刺激其扩增,流式细胞术测定其纯度和表型。以CD4+CD25-T细胞作为反应细胞,验证扩增前后Treg细胞的免疫抑制功能。结果MACS分离的CD4+CD25+调节性T细胞纯度达到(95.38±1.82)%,同基因和异基因DC都能有效刺激Treg细胞体外扩增,其中同基因成熟DC扩增效果最为明显。而且同基因成熟DC扩增后CD4+CD25+调节性T细胞纯度达到(94.16±1.88)%,而且高表达Foxp3分子。当CD4+CD25+调节性T细胞与效应T细胞比例为1∶1时,能够有效的抑制效应T细胞的增殖,而且,同基因成熟DC扩增的CD4+CD25+调节性T细胞的抑制效果比新分离的Treg效果更好。结论同基因成熟DC能够体外扩增表型和功能稳定的Treg细胞。
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| 关 键 词： | CD4+CD25+Foxp3+ 调节性T细胞 同基因/异基因 树突状细胞 体外扩增 增殖抑制 |
Comparison of CD4+CD25+ Foxp3+ regulatory T cells expanded by different dendritic cells |
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| FENG Tao,YANG Jie,XIE Rufeng,et al..Comparison of CD4+CD25+ Foxp3+ regulatory T cells expanded by different dendritic cells[J].Chinese Journal of Blood Transfusion,2009,22(4). |
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| Authors: | FENG Tao YANG Jie XIE Rufeng |
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| Institution: | FENG Tao1,2,YANG Jie2,XIE Rufeng2,et al.1 Department of Biomedical Science,School of Life Sciences,East China Normal University,Shanghai 200062,China,2 Laboratory for Blood Engineering,Shanghai Blood Center |
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| Abstract: | Objective To expand CD4+CD25+ Foxp3+ regulatory T cells(Treg cells) with stable phenotype and function by using different dendritic cells(DC).Methods CD4+CD25+ Foxp3+ regulatory T cells were purified from splenocyte(Balb/c) with immunomagnetic beads(MACS),and were expanded using homologous or heterologous mature DC,immature DC and regulatory DC.The phenotype and purity of Treg cells were determined by flow cytometry.Immunosuppressive function of Treg cells were verified using CD4+CD25-T cells.Results The pu... |
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| Keywords: | CD4+CD25+ Foxp3+ regulatory T cells Homologous or heterologous mature DC Expand ex vivo Proliferation suppression |
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