| HPLC测定翅茎白粉藤中白黎芦醇的含量 |
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| 引用本文： | 刘元,宋志钊,文志云. HPLC测定翅茎白粉藤中白黎芦醇的含量[J]. 中国现代应用药学, 2012, 29(8): 734-736 |
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| 作者姓名： | 刘元 宋志钊 文志云 |
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| 作者单位： | 广西中医药研究院，南宁 530022 |
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| 基金项目： | 广西科技攻关项目(桂科攻0992003A-22)；广西壮药质量标准(第二卷)标准研究项目(MZY2010054)；广西壮族自治区卫生厅自筹经费科研课题(Z2011276) |
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| 摘 要： | 目的建立翅茎白粉藤中白黎芦醇的含量测定方法。方法采用高效液相色谱法,色谱柱XB-C18(4.6 mm×250 mm,5μm),以乙腈-水(25∶75)为流动相,检测波长为306 nm,柱温为室温。结果白黎芦醇在2.50~40.0μg内峰面积与其浓度呈良好线性关系(r=0.999 9),平均回收率为97.3%,RSD为0.98%(n=6)。结论本方法简便、准确、重复性好,可作为该药材含量测定方法。
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| 关 键 词： | 翅茎白粉藤 高效液相色谱法 白黎芦醇 |
| 收稿时间： | 2011-12-09 |
| 修稿时间： | 2012-03-16 |
Determination of Resveratrol in Cissus Hexangularis by HPLC |
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| LIU Yuan,SONG Zhizhao and WEN Zhiyun. Determination of Resveratrol in Cissus Hexangularis by HPLC[J]. The Chinese Journal of Modern Applied Pharmacy, 2012, 29(8): 734-736 |
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| Authors: | LIU Yuan SONG Zhizhao WEN Zhiyun |
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| Affiliation: | Guangxi Institute of Chinese Medicine & Pharmaceutical Scince, Nanning 530022, China |
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| Abstract: | OBJECTIVE To develop an HPLC method for detecting resveratrol in Cissus hexangularis. METHODS An XB-CIg column（4.6 mm×250 mm, 5 μm） was used. The mobile phase consisted of acetonitrile-water（25 ： 75）. The detection wavelength was 306 nm and the column temperature was room temperature. RESULTS Resveratrol showed a good linear relationship in the range of 2.50-40.0 μg.mL^-1, r=0.999 9. The recovery and RSD were 97.5% and 0.98%（n=6）, respectively. CONCLUSION This method is simple, accurate and repeatable to determine the content ofresveratrol in Cissus hexangularis. |
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| Keywords: | Cissus hexangularis HPLC resveratrol |
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