菊花提取物中总黄酮与总有机酸的含量测定

目的建立菊花提取物总黄酮与总有机酸含量测定方法。方法采用UV法,以木犀草素为对照,在268 nm处测定菊花提取物总黄酮含量。采用硝酸铝显色反应,可见分光光度法在510 nm处测定菊花提取物总酚含量。总有机酸含量为总酚含量与总黄酮含量之差。将上述含量测定方法的结果与HPLC含量测定结果进行了比较。结果本研究方法测定菊花提取物总黄酮、总酚和总有机酸含量结果与HPLC法含量测定结果相近。采用UV法,木犀草素质量浓度在4.75～38.00 mg.L-1内,线性关系良好,相关系数为1.000 0,平均回收率为101.2%(RSD=2.3%);采用可见分光光度法,木犀草素质量浓度在1.90～30.40 mg.L-1内,线性关系良好,相关系数为0.999 9,平均回收率为99.4%(RSD=2.4%)。结论该方法简便、准确、重现性好,可用于菊花提取物质量控制。不同厂家商品菊花提取物总黄酮与总有机酸含量差异较大。

Quantitative determination of the total flavonoids and organic acids of Chrysanthemum morifolium extract

Objective To establish quantitative methods of total flavonoids and total organic acids of Chrysanthemum morifolium extract. Methods The content of total flavonoids was determined at 268 nm by UV method using luteolin as standard., and that of total phenols was done at 510 nm by Vis spectrophotometry using the color reaction with Al(NO₃)₃. The content of total organic acids is the value for the differences between the content of total phenols and that of total flavonoids. The results obtained with quantitative methods mentioned above were compared with those with HPLC method. Results The content of total flavonoids, total organic acids and total phenols with UV-Vis method were consistent with those with HPLC method. The linear range for the UV determination was 4.75-38.00 mg·L^-1, with correlation coefficient being 1.000 and the recovery being 100.17% (RSD=2.29%). The linear range for the Vis determination was 3.80-30.40 mg·L^-1, with correlation coefficient being 0.999 9 and the recovery being 99.36% (RSD=2.36%). Conclusion The present methods are simple, accurate with good reproducibility, suggesting they are practical for the quality control of Chrysanthemum morifolium extract. There is a significant difference in the contents of total flavonoids and total organic acids of Chrysanthemum morifolium extract from different makers.