Bioactive starch-based scaffolds and human adipose stem cells are a good combination for bone tissue engineering |
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| Authors: | Rodrigues A I Gomes M E Leonor I B Reis R L |
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| Institution: | 1. Polymeric Materials Research Group, Department of Materials Science and Engineering, Sharif University of Technology, Tehran, P.O. Box 11155-9466, Iran;2. Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran;1. Department of Physics, University of Warwick, Coventry CV4 7AL, UK;2. Coe College, Cedar Rapids, Iowa 52402, USA;3. AWE, Aldermaston, Berkshire, UK;2. Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, University Hospital Münster, Germany;1. Division of Endocrinology, Diabetes, and Bone Diseases, Department of Medicine III, Dresden Technical University Medical Center, D-01307 Dresden, Germany;2. Department of Proteomics, Helmholtz-Centre for Environmental Research, D-04318 Leipzig, Germany;3. Department of Metabolomics, Helmholtz-Centre for Environmental Research, D-04318 Leipzig, Germany;4. Biomaterials Department, INNOVENT e.V., D-07745 Jena, Germany;5. Jena Center for Soft Matter, Jena, Germany;6. Institute of Materials Science, Max Bergmann Center for Biomaterials, Technische Universität Dresden, D-01069 Dresden, Germany;7. Center for Regenerative Therapies Dresden, Dresden, Germany;1. Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, Shantou, Guangdong Province, China;2. Department of Pathology, Cancer Hospital of Shantou University Medical College, Shantou, Guangdong Province, China;3. Department of Radiation Oncology, Stanford University, Stanford, California;4. Department of Pathology, Sun Yat-Sen University Cancer Center, Guangzhou, Guangdong Province, China;6. Department of Radiation Oncology, Chaozhou Hospital of Chaozhou City, Guangdong Province, China;5. Department of Pathology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong Province, China |
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| Abstract: | Silicon is known to have an influence on calcium phosphate deposition and on the differentiation of bone precursor cells. This study explores the effect of the incorporation of silanol (Si-OH) groups into polymeric scaffolds on the osteogenic differentiation of human adipose stem cells (hASC) cultured under dynamic and static conditions. A blend of corn starch with polycaprolactone (30/70wt.%, SPCL) was used to produce three-dimensional fibre meshes scaffolds by the wet-spinning technique, and a calcium silicate solution was used as a non-solvent to develop an in situ functionalization with Si-OH groups. In vitro assessment, using hASC, of functionalized and non-functionalized scaffolds was evaluated in either α-MEM or osteogenic medium under static and dynamic conditions (provided by a flow perfusion bioreactor). The functionalized materials, SPCL-Si, exhibit the capacity to sustain cell proliferation and induce their differentiation into the osteogenic lineage. The formation of mineralization nodules was observed in cells cultured on the SPCL-Si materials. Culturing under dynamic conditions using a flow perfusion bioreactor was shown to enhance the hASC proliferation and differentiation and a better distribution of cells within the material. The present work demonstrates the potential of these functionalized materials for future applications in bone tissue engineering. Additionally, these results highlight the simplicity, economic and reliable production process of those materials. |
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