| Epstein-Barr病毒壳抗原gp125的纯化和应用 |
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| 引用本文： | 王桂敏,皮国华,谷淑燕.Epstein-Barr病毒壳抗原gp125的纯化和应用[J].中华实验和临床病毒学杂志,1998,12(2):143-146. |
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| 作者姓名： | 王桂敏 皮国华 谷淑燕 |
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| 作者单位： | 中国预防医学科学院病毒学研究所 |
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| 摘 要： | 目的研制ｅｐｓｔｅｉｎ－Ｂａｒ（ＥＢ）病毒诊断试剂。方法将重组痘苗病毒表达的Ｅｐｓｔｅｉｎ－Ｂａｒ病毒（ＥＢＶ）壳抗原（ＶＣＡ）主要多肽ｇｐ１２５纯化，作为诊断抗原建立了酶联免疫吸附试验（ＥＬＩＳＡ），检测了４８份鼻咽癌（ＮＰＣ）病人血清及１０份正常人血清中的ＶＣＡ／ＩｇＡ抗体。结果该方法与免疫荧光（ＩＦ）检测结果一致，但ＥＬＩＳＡ的平均几何滴度（ＧＭＴ）是ＩＦ的１２倍。结论以纯化的ＥＢ病毒壳抗原主要多肽ｇｐ１２５作为诊断抗原建立的检测方法，更适合于ＥＢＶ相关疾病的血清学诊断和血清流行病学调查。
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| 关 键 词： | Epstein－Bar病毒 病毒抗原 血清学诊断 酶联免疫吸附测定 |
The purification and application of Epstein-Barr virus gp125 |
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| G Wang,G Pi,S Gu.The purification and application of Epstein-Barr virus gp125[J].Chinese Journal of Experimental and Clinical Virology,1998,12(2):143-146. |
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| Authors: | G Wang G Pi S Gu |
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| Institution: | Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052. |
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| Abstract: | Epstein-Barr virus (EBV) virion capsid antigen (VCA) gp125 as a diagnostic antigen was expressed in infected cells by recombinant vaccinia virus. The presence of IgA antibody to VCA of EBV was tested by ELISA in sera from nasopharyngeal carcinoma (NPC) patients and normal individuals. The ELISA consisted of three steps involving test serum, mouse monoclonal antibody to human IgA and rabbit anti-mouse IgG. The antibody titers of sera determined by this way correlated well with those by IF. The GMT by ELISA was 12 times higher than that by IF. Furthermore, efforts to develop gold-conjugated immunosorbent assay (GCISA), which is a more sensitive assay for measuring IgA antibody to VCA, has met with initial results. |
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| Keywords: | Epstein-Barr virus Antigen viral Serodiagnosis Enzyme-linked immunosorbent assay |
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