深圳地区不同人群TTV感染情况的调查

目的了解深圳地区不同人群ＴＴＶ的感染情况。方法在ＴＴＶＯＲＦ１保守区设计两对套式引物，建立了检测ＴＴＶＤＮＡ的巢式聚合酶链反应（Ｎｅｓｔｅｄ－ＰＣＲ），用该法对深圳地区９０例一般人群、８８例职业献血员、７９例静脉毒瘾者及２９例非甲庚型肝炎病人进行ＴＴＶＤＮＡ的检测。结果ＴＴＶＤＮＡ在以上４种人群中的阳性率分别为７８％，９０％，４１７％与４４８％，前者与后两者比较差异均有显著性（Ｐ＜００５）。９０例一般人群与８８例职业献血员中，１４例ＴＴＶＤＮＡ阳性者丙氨酸转氨酶（ＡＬＴ）均正常。结论深圳地区一般人群与职业献血员中ＴＴＶ携带者较常见；静脉毒瘾者是ＴＴＶ感染的高危人群；部分非甲～庚型肝炎可能与ＴＴＶ相关

Investigation on TT virus infection among various populations in shenzhen

A polymerase chain reaction assay with nested primers (nested PCR) deduced from the well conserved sequences of TTV ORF1 region was established to detect TTV DNA. PCR products from the 2 patients with non A-G hepatitis were cloned and sequenced to confirm its specificity. By using this assay, we tested the serum samples of 90 normal individuals, 88 paid blood donors, 79 intravenous drug users and 29 patients with non A-G hepatitis in Shenzhen, China. The positive rates of TTV DNA were 7 8%,9 0%,41 7% and 44 8%, respectively. TTV DNA was detected in 14 of the 90 normal individuals and 88 paid blood donors without any symptoms of liver disease. The results of this study suggest that TTV infection is common in normal population and the paid blood donors. The intravenous drug users are at high risk for TTV infection. TTV would be responsible for a part of non A-G hepatitis.