不同慢性肝病患者血清中乙型肝炎病毒DNA定量检？…

目的 探讨乙型肝炎病毒ＤＮＡ含量的临床意义。了解乙型肝炎病毒（ＨＢＶ）免疫标志不同状态的慢性肝病患者血清ＨＢＶＤＮＡ浓度及共临床意义。方法 应用建立的竞争性聚合酶链反应（ＰＣＲ）方法定量检测慢性肝炎（ＣＨ）５１例、肝硬化（ＬＣ）３６例、原发性肝癌（ＰＨＣ）３８例的血清ＨＢＶ ＤＮＡ浓度。结果 ＨＢＶＤＮＡ阳性的ＣＨ患者血清ＨＢＶ ＤＮＡ浓度为４．３６ｌｏｇ１０ＨＢＶＤＮＡ拷贝／５０μｌ，ＬＣ为４．

Detection of serum hepatitis B virus DNA in patients with various chronic liver diseases]

With established competitive polymerase chain reaction (PCR) method, serum HBV DNA concentration was determined in 125 patients with primary hepatic carcinoma(n = 38), liver cirrhosis (n = 36) and chronic hepatitis (n = 51) with different serum HBV immunological markers. The results showed that: (1) No significant variation of HBV DNA concentration was detected in patients with different chronic liver diseases; (2) About one third of the examined patients whose five serum HBV markers were negative and anti-HBs were positive showed low level of viral replications; (3) The total concentration of HBV DNA in the patients with positive HBeAg were significantly higher than that in patients positive for anti-HBe, but also found a high HBV DNA concentration in some of the latter. It suggests that the manner of viral replication were not correlated with the period of the chronic liver disease. So HBV replicative status may not be completely determined only by seroconvertion of HBeAg to anti-HBe.