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Quantification by ELISA of Aldrin/Dieldrin in river Nile water and tap water samples collected in Egypt
Authors:A A Ragab  A M A Ibrahim  C J Smith
Institution:1. Department of Biochemistry, Faculty of Agriculture , Cairo University , Cairo, Egypt;2. Department of Dairy Technology, Faculty of Agriculture , Cairo University , Fayoum, Egypt;3. CORTECS Diagnostics Ltd , Newtech Square, Deeside Industrial Park, Clwyd, CH52NT, UK
Abstract:An indirect competitive ELISA was developed for the quantitative analysis of aldrin/dieldrin. This has been used to monitor the levels of these pesticides in water samples collected in Egypt. The detection range of the aldrin/dieldrin assay in water was 5–10 ppm without enhancement. River Nile water samples and tap water samples (n = 25) were collected from 25 stations in 16 different governorates in Egypt. Pesticides were detected in 10 of the 25 Nile water samples (40%) at levels from 10 to 110 ppb. In tap water samples, pesticides were not detected at levels greater than the lower limit of the assay. The technique described provides a rapid, economical, highly sensitive and specific method of analysis that is relatively simple to perform and interpret. It will be extremely useful in monitoring levels of pesticides in water used for human consumption.
Keywords:ELISA  aldrin  dieldrin  water  Egypt
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