Chymotrypsin treatment increases the activity of Clostridium perfringens enterotoxin |
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Authors: | P E Granum M Richardson |
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Institution: | Department of Food Hygiene, Norwegian College of Veterinary Medicine, Oslo. |
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Abstract: | Chymotrypsin treatment of the enterotoxin from Clostridium perfringens results in the loss of 36 amino acids from the N-terminus and 3 amino acids from the C-terminus. This processing results in a 3.2 fold increase in activity on Vero cells, which is close to what had been found for trypsin treatment following the loss of 25 amino acids from the N-terminus. |
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