灯盏花素对大鼠出血性脑损伤的保护作用

目的:探讨灯盏花素注射液对大鼠出血性脑损伤的保护作用。方法:用胶原酶和肝素混合液注入大鼠尾壳核建立脑出血(ICH)模型,然后将大鼠随机分为对照组、模型组和治疗组。治疗组动物于注射灯盏花素注射液后第3、5、7 d后处死。应用Berdson评分标准对各组大鼠进行神经功能评分,应用免疫组织化学方法检测出血灶周围TNF-α和IL-8的表达,应用流式细胞术检测神经细胞凋亡率。结果:与模型组比较,治疗组大鼠神经功能评分明显增加(P<0.05);灯盏花素治疗组脑细胞的凋亡率明显减低(P<0.05);治疗组出血灶周围炎症因子TNF-α,IL-8明显减少(P<0.05);结论:灯盏花素注射液能保护神经元,促进神经功能恢复。

The protective effect of erigeron on hemorrhagic brain injury in rats

Objective: To explore the protective effect of Erigeron injection on hemorrhagic brain injury in rats.Methods: The intracerebral hemorrhage(ICH) model was established in rats with a mixture of collagenase and heparin injected into caudate-putamen,and then the rats were randomly divided into three groups:control,model and treatment groups,respectively.Animals of treatment group were killed on the 3rd,5th and 7th day after drug injection.Neurological functional scores of each group were evaluated according to the Berdson Scoring Criteria.The expression of TNF-α and IL-8 in brain areas around hemorrhagic focus were tested by immunohistochemical methods,and apoptosis rate of nerve cells was examined by flow cytometry.Results: Compared with the model group,neurologic function scores of treatment group were significantly increased(P<0.05).Nerve cell apoptosis rate of Erigeron-treated group was significantly decreased(P<0.05);and inflammatory factors,TNF-α and IL-8 in brain areas around hemorrhagic focus were significantly decreased in treatment group(P<0.05).Conclusion: Erigeron injection can protect neurons and promote the recovery of neurological function.