沈阳市1份人类免疫缺陷病毒的基因序列分析

目的 :对 2 0 0 4年我院门诊检出的 1例艾滋病感染者体内的HIV 1病毒进行基因序列分析和亚型鉴定。方法 :从HIV 1感染者的全血标本中提取脱氧核糖核酸 (DNA)作为PCR扩增的模板 ,套式引物扩增HIV 1前病毒DNA的p17与p2 4交界部分的基因片断并进行测序。所测定序列与各亚型国际参考株及亚洲流行参考序列进行比较 ,确定被检标本的亚型 ,并进行基因序列分析。结果 :该患HIV 1病毒属于B’亚型毒株 ,经输供血途径感染。该毒株与泰国B’亚型参考株B TH 92 92TH0 14的基因距离最为接近。结论 :在沈阳市有效地控制艾滋病经输供血途径传播应引起我们高度的重视

Gene Sequence Analysis on HIV-1 Virus of One Infected Individual Found in Shenyang City

Objective:To analyze variation of gene sequence and identify genetic subtype of HIV-1 virus of 1 infected individual found in the central hospital affiliated to Shenyang Medical College.Methods:Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive anti-retrovirus therapy naive patient.DNA fragments of HIV-1 p17/p24 gene were amplified by nested-PCR from provirus in human genome. The amplified products of p17/p24 region of HIV-1 were sequenced and compared with reference HIV-1 strains of different kinds of HIV-1 genetic subtype and some strains epidemic in Asia. The analysis of the gene-based phylogenetic tree and variation of amino acid were carried out. Results:This sample was positive by PCR amplification of HIV-1 gag region and found to be genetic subtype B'. This person was blood recipient and blood donor. The HIV-1 subtype B' strain is most close to Thailand strain B.TH.92.92TH014. Conclusion:There has been B' subtype of HIV-1 existed in Shenyang city. We should pay much attention to complexity of HIV epidemiology by blood route in Shenyang city and the difficulty conducting prevention and treatment.