Immunoblotting analysis of concanavalin A -isolated allergens of Candida albicans |
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Authors: | J. Savolainen M. Viander R. Einarsson A. Koivikko |
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Affiliation: | Department of Medical Microbiology, University of Turku, Finland. |
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Abstract: | The carbohydrate-containing fraction of Candida albicans was isolated from the crude extract with ConA Sepharose affinity chromatography and studied by IgE-immunoblotting with individual and pooled sera from C. albicans-allergic subjects. In the ConA-bound fraction there was a diffuse IgE binding in the high molecular weight area which also gave a carbohydrate stain (PAS). A distinct band corresponding to a molecular weight of 70 kD bound specific IgE antibodies. This glycoprotein, presumably a mannoprotein, gave a weak carbohydrate staining and a strong protein staining. Further biochemical studies are needed to reveal the exact nature of the epitopes in the ConA-bound mannose-containing fraction of C. albicans. |
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Keywords: | allergen Candida albicans glycoproteins IgE immunoblotting mannans |
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