| 复制缺陷型鼠IL-3重组腺病毒的构建和鉴定 |
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| 引用本文： | 刘林,罗成基,袁良平.复制缺陷型鼠IL-3重组腺病毒的构建和鉴定[J].免疫学杂志,1999,15(4):242-244. |
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| 作者姓名： | 刘林 罗成基 袁良平 |
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| 作者单位： | 第三军医大学新桥医院血液科!重庆400037(刘林)，第三军医大学复合伤研究所(罗成基，袁良平，粟永平) |
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| 摘 要： | 构建以ＣＭＶ启动子控制鼠ＩＬ－３基因表达的复制缺陷型腺病毒（ＡｄＣＭＶＩＬ－３）。将含全部编码序列的鼠ＩＬ－３ｃＤＮＡ克隆于腺病毒穿梭质粒ｐＭＨ４的ＣＭＶ启动子下游，再与含腺病毒基因组的质粒ｐＢＨＧ１０共转染２９３细胞，经细胞内同源重组后产生７个病毒空斑，取其中４个空斑扩增后经双引物ＰＣＲ检测，结果均含有ＩＬ－３及腺病毒特有片段，表明成功地构建了携带鼠ＩＬ－３基因的复制缺陷型重组腺病毒。
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| 关 键 词： | 白细胞介素3 腺病毒载体 基因治疗 |
CONSTRUCTION AND IDENTIFICATION OF REPLICATION DEFICIENT MOUSE INTERLEUKIN 3 RECOMBINANT ADENO VIRUSES |
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| Liu,Lin.CONSTRUCTION AND IDENTIFICATION OF REPLICATION DEFICIENT MOUSE INTERLEUKIN 3 RECOMBINANT ADENO VIRUSES[J].Immunological Journal,1999,15(4):242-244. |
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| Authors: | Liu Lin |
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| Abstract: | The full length mouse IL 3 encoding cDNA was cloned at the downstream of CMV promoter of the adenoviral shuttle plasmid pMH4,and the resultant pMH4 IL 3 was cotransfected into 293 cells together with the plasmid pBHG10 carried the adenoviral genome.Among 7 plaques generated by homologous recombination,4 plaques were picked out and identified by PCR co amplification.The result showed that every plaque had both the fragment of IL 3 and the special fragment of adenoviral genome.These data suggest that the replication deficient mouse IL 3 recombinant adenoviruses have been constructed successfully. |
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| Keywords: | Interleukin 3(IL 3) Adenovirus vector Gene therapy |
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