高血压性脑出血与血管紧张素转换酶基因多态性及血清水平关系的研究

目的：探讨原发性高血压（EH），高血压性脑出血（CH）与血管紧张素转换酶（ACE）基因I／D多态性及血清ACE水平的相互关系。方法：对正常人（NC组）29例，EH组28例和CH组31例提取白细胞DNA，检测ACE基因型，等位基因和血清水平。结果：88例中不同ACE基因型血清ACE水平有显著性差异（DD＞ID＞II，P＜0．01），EH组DD基因及D基因频率与NC组比较无显著性差异（P＜0．05），CH组血清ACE水平和D基因频率显著高于NC组及EH组（P＜0．01），其DD型的血清ACE水平也高于后二者（P＜0．05），结论：ACE基因多态性及其血清水平与EH无关，而与CH呈正相关，D基因可能为高血压病患者脑出血发病的相对危险因素。

Gene polymorphism and serum levels of the angiotensin-converting enzyme (ACE) in relation to cerebral hemorrhage in hypertensives

Objective To investigate the distribution of angiotensin-converting enzyme (ACE) genotype and serum ACE levels in cases of essential hypertension with and without cerebral hemorrhage. Methods By using Nal and polymerase chain reaction (PCR), the ACE genotype and allele were identified in 28 patients with essential hypertension (EH) and 31 cerebral hemorrhage with hypertension (CH), compared with 29 normal control (NC).Meanwhile, serum ACE levels were determined by hydrolysis of hippuryl-glycylglycine (HGG). Results Compared with their frequencies in EH and NC, the D allele frequency was significantly higher ( P ＜ 0.01 ). Whereas the ACE genotype and allele frequencies were apparently not different between EH and NC (P ＞0.05). A marked difference in serum ACE levels was observed among all 88 subjects in the three ACE genotype classes (DD ＞ ID ＞ Ⅱ , P ＜0.01 ). Conclusion There is a negative association of ACE gene polymorphism and serum ACE levels with essential hypertension, while a deletion polymorphism and ascending serum levels are related to cerebral hemorrhage with hypertension. The D allele is a relative risk factor of morbidity of cerebral hemorrhage in hypertensives.