组胺和类胰蛋白酶在大鼠慢性胰腺炎疼痛中的作用

目的探讨组胺和类胰蛋白酶在慢性胰腺炎(CP)疼痛发生与维持中的作用。方法制备CP模型大鼠,检测模型组和对照组大鼠胰腺标本中的组胺和类胰蛋白酶含量并进行比较;然后将模型组和对照组大鼠胰腺标本孵育上清分别注射于正常大鼠足底,观察注射后0.5、1、3、5、7h大鼠痛觉阈值变化;以模型组大鼠胰腺标本孵育上清注射正常大鼠足底,并给予H1受体阻断剂和蛋白酶抑制剂,观察其对痛敏的治疗作用。结果 CP模型大鼠的胰腺局部组胺及类胰蛋白酶分泌量较对照组增高(P<0.05),其标本孵育上清在注射后5h内有明显的痛觉致敏作用,且该作用可被H1受体阻断剂和蛋白酶抑制剂所逆转。结论组胺和类胰蛋白酶是CP顽固性疼痛发生维持中的重要介质。

Roles of histamine and tryptase in chronic pancreatitis pain in rats

Objective To explore the roles of histamine and tryptase in chronic pancreatitis pain development and maintenance. Methods Rat models of chronic pancreatitis were prepared, and the histamine and tryptase contents were examined in the pancreatic specimens of both model group and the control group. The pancreas incubation supernatants of both groups were injected into the plantar surface of the rat hindpaw, and then the pain thresholds were observed at 0.5, 1, 3, 5, and 7 h after injection. Rats injected with the supernatant of chronic pancreatitis model were also given H1 receptor blocker or protease inhibitor to observe their treatment for hyperalgesia. Results The histamine and tryptase contents in the pancreas specimens were increased in chronic pancreatitis model rats. Injection of supernatant from the chronic pancreatitis specimens had a profound hyperalgesia effect within 5 h after injection, and the effect could be partially reduced by H1 receptor blockers and protease inhibitors. Conclusion Our findings suggest that histamine and tryptase are important mediators in the development and maintenance of the chronic pancreatitis pain.